Anti-Dynein light chain antibody (ab236594)
Key features and details
- Rabbit polyclonal to Dynein light chain
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Dynein light chain antibody
See all Dynein light chain primary antibodies -
Description
Rabbit polyclonal to Dynein light chain -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Pig
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Immunogen
Recombinant full length protein corresponding to Human Dynein light chain aa 1 to the C-terminus.
Database link: O96015 -
Positive control
- WB: Mouse gonadal and brain lysates; HL-60 and NIH/3T3 whole cell lysates. IHC-P: Human testis and breast cancer tissues. ICC/IF: MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Dynein light chain antibody (ab236594)All lanes : Anti-Dynein light chain antibody (ab236594) at 1/500 dilution
Lane 1 : Mouse gonadal lysate
Lane 2 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lane 3 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lane 4 : Mouse brain lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 12 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynein light chain antibody (ab236594)Paraffin-embedded human testis tissue stained for Dynein light chain using ab236594 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynein light chain antibody (ab236594)Paraffin-embedded human breast cancer tissue stained for Dynein light chain using ab236594 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Dynein light chain antibody (ab236594)MCF7 (human breast adenocarcinoma cell line) cells stained for Dynein light chain (green) using ab236594 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
