Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cerebral cortex tissue sections from Alzheimer's disease patients labeling Artemin with ab205564 at 5 µg/mL. DAB was used for visualization. Sections were counterstained with toluidine blue.

Immunofluorescence analysis of GFRA3-Ret51 receptor complex bound Artemin. Human U2OS cells were transfected with GFRa3-Ret51 expression vector followed by treatment with 100 nM Artemin. Artemin bound to the GFRa3-Ret51 receptor complex was detected with ab205564 at 20 µg/mL. Goat anti-mouse Dylight 488 conjugated antibody (1 µg/mL) was used as secondary antibody. For nuclear staining DAPI was used. Left image; Artemin bound to the GFRa3-Ret51 receptor complex; Right image; Negative control - GFRa3-Ret51 receptor expressing U2OS cells.