Recombinant human PSPN protein (Active) (ab218092)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human PSPN protein (Active)
See all PSPN proteins and peptides -
Biological activity
Determined by TT cell proliferation assay ED50 ≤20 ng/mL (≥ 5.0 x 104 units/mg) (lot specific).
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Purity
> 95 % SDS-PAGE. -
Endotoxin level
Expression system
Escherichia coliAccession
Protein length
Protein fragmentAnimal free
NoNature
Recombinant-
Species
Human -
Sequence
MALSGPCQLW SLTLSVAELG LGYASEEKVI FRYCAGSCPR GARTQHGLAL ARLQGQGRAH GGPCCRPTRY TDVAFLDDRHRWQRLPQLSA AACGCGG -
Predicted molecular weight
10 kDa -
Amino acids
61 to 156
Specifications
Our Abpromise guarantee covers the use of ab218092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 0.16% Sodium phosphate
0.2 micron filteredThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute in Sterile water at 0.1 mg/mL. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
General Info
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Alternative names
- Persephin
- PSP
- PSPN
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Relevance
PSPN is a neurotrophic factor, belonging to the GDNF family. Neurotrophic factors are important for the proper development and maintenance of the nervous system. These factors promote neuronal survival and can prevent the neuronal degeneration associated with injury, toxin exposure, or neurodegenerative disease. PSPN has a similar structure to other family members, glial cell line-derived neurotrophic factor, Artemin and neurturin. All these proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. PSPN signals through a multicomponent receptor system, composed of RET and one of four GFR alpha (alpha1-alpha4) receptors. The GFRalpha4 was first identified in chicken and was later shown to be the preferential binding subunit for PSPN. In contrast to GDNF and Neurturin, PSPN does not support survival of peripheral neurons. -
Cellular localization
Secreted
Images
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SDS-PAGE - Recombinant human PSPN protein (Active) (ab218092)
SDS-PAGE analysis of ab218092 (1 μg) stained with Coomassie blue:
Lane 1: Non-reduced
Lane 2: Reduced
4-20% Tris-Glycine gel
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab218092 has not yet been referenced specifically in any publications.
Preparation and Storage
- Persephin
- PSP
- PSPN
Images
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SDS-PAGE - Recombinant human PSPN protein (Active) (ab218092)
SDS-PAGE analysis of ab218092 (1 μg) stained with Coomassie blue:
Lane 1: Non-reduced
Lane 2: Reduced
4-20% Tris-Glycine gel
