Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Extracellular matrix protein 1 antibody [EPR6701] - BSA and Azide free (ab242391)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6701] to Extracellular matrix protein 1 - BSA and Azide free
Suitable for: ICC/IF, IHC-P, IP, WB, Flow Cyt
Reacts with: Rat, Human

Product name

Anti-Extracellular matrix protein 1 antibody [EPR6701] - BSA and Azide free
See all Extracellular matrix protein 1 primary antibodies
Description

Rabbit monoclonal [EPR6701] to Extracellular matrix protein 1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, IHC-P, IP, WB, Flow Cytmore details
Species reactivity

Reacts with: Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human breast carcinoma and Human kidney tissues. ICC/IF: A375 cells. IP: A375 cells. Flow Cyt: A375 cells.
General notes
ab242391 is the carrier-free version of ab126629 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab242391 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6701
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] - BSA and Azide free (ab242391)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Extracellular matrix protein 1 with purified ab126629 at 1:100 dilution (2.01 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR6701] - BSA and Azide free (ab242391)

ab126629 (purified) at 1:20 dilution (2µg) immunoprecipitating Extracellular matrix protein 1 in A375 whole cell lysate.
Lane 1 (input): A375 (Human malignant melanoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab126629 & A375 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126629 in A375 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Extracellular matrix protein 1 antibody [EPR6701] - BSA and Azide free (ab242391)

Immunocytochemistry/ Immunofluorescence analysis of A375 (Human malignant melanoma epithelial cell) cells labeling Extracellular matrix protein 1 with purified ab126629 at 1:100 dilution (2.0 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry - Anti-Extracellular matrix protein 1 antibody [EPR6701] - BSA and Azide free (ab242391)

Flow Cytometry analysis of A375 (Human malignant melanoma epithelial cell) cells labeling Extracellular matrix protein 1 with purified ab126629 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] - BSA and Azide free (ab242391)

ab126629, at 1/100, staining Extracellular matrix protein 1 in formalin fixed, paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126629).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] - BSA and Azide free (ab242391)

ab126629, at 1/100, staining Extracellular matrix protein 1 in formalin fixed, paraffin embedded Human kidney tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126629).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
OI-RD Scanning - Anti-Extracellular matrix protein 1 antibody [EPR6701] - BSA and Azide free (ab242391)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126629).
Anti-Extracellular matrix protein 1 antibody [EPR6701] - BSA and Azide free (ab242391)

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