Recombinant human Nectin 3 protein (Fc Chimera Active) (ab221336)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
- CD113
- CDw113
- DKFZP566B0846
Images
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Functional Studies - Recombinant human Nectin 3 protein (Fc Chimera Active) (ab221336)
Immobilized Human TIGIT at 1 μg/mL (100 μL/well) can bind ab221336 with a linear range of 0.156-2.5 μg/mL.
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SDS-PAGE - Recombinant human Nectin 3 protein (Fc Chimera Active) (ab221336)Human Nectin-3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%. The protein migrates as 80-95 kDa under reducing conditions due to glycosylation.
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Functional Studies - Recombinant human Nectin 3 protein (Fc Chimera Active) (ab221336)Loaded ab221336 on Protein A Biosensor, can bind Human TIGIT with an affinity constant of 0.732 μM as determined in BLI assay (ForteBio Octet Red96e).
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Functional Studies - Recombinant human Nectin 3 protein (Fc Chimera Active) (ab221336)Immobilized Human Nectin-1, His Tag at 2 µg/mL (100 µL/well) can bind ab221336 with a linear range of 5-78 ng/mL.
