Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6701] to Extracellular matrix protein 1
  • Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Extracellular matrix protein 1 antibody [EPR6701]
    See all Extracellular matrix protein 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6701] to Extracellular matrix protein 1

  • Host species

    Rabbit

  • Specificity

    The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Extracellular matrix protein 1 aa 100-200. The exact sequence is proprietary.
    Database link: Q16610

  • Positive control

    • WB: MDA MB 435 cell line, Rat heart, Rat kidney, Human skin cell, and A375 cell line lysates. IHC-P: Human breast carcinoma and Human kidney tissues. ICC/IF: A375 cells. IP: A375 cells. Flow Cyt: A375 cells.

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Extracellular matrix protein 1 with purified ab126629 at 1:100 dilution (2.01 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
    Western blot - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
    All lanes : Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629) at 1/2000 dilution (Purified)

    Lane 1 : A375 (Human malignant melanoma epithelial cell)
    Lane 2 : Rat heart lysates
    Lane 3 : Rat kidney lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 61 kDa
    Observed band size: 61 kDa

  • Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
    Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)

    ab126629 (purified) at 1:20 dilution (2µg) immunoprecipitating Extracellular matrix protein 1 in A375 whole cell lysate.
    Lane 1 (input): A375 (Human malignant melanoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab126629 & A375 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126629 in A375 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
    Immunocytochemistry/ Immunofluorescence - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
    Immunocytochemistry/ Immunofluorescence analysis of A375 (Human malignant melanoma epithelial cell) cells labeling Extracellular matrix protein 1 with purified ab126629 at 1:100 dilution (2.0 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
    Flow Cytometry - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
    Flow Cytometry analysis of A375 (Human malignant melanoma epithelial cell) cells labeling Extracellular matrix protein 1 with purified ab126629 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)

    ab126629, at 1/100, staining Extracellular matrix protein 1 in formalin fixed, paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
    Western blot - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
    All lanes : Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629) at 1/1000 dilution

    Lane 1 : MDA MB 435 cell line lysate
    Lane 2 : Human skin cell lysate
    Lane 3 : A375 cell line lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibodty at 1/2000 dilution

    Predicted band size: 61 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)

    ab126629, at 1/100, staining Extracellular matrix protein 1 in formalin fixed, paraffin embedded Human kidney tissue by Immunohistochemistry.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
    OI-RD Scanning - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)
    Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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