Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free (ab267392)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22411-279] to Extracellular matrix protein 1 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IP
  • Knockout validated
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free
    See all Extracellular matrix protein 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22411-279] to Extracellular matrix protein 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 4T1, LLC1, Hepa1-6 whole cell lysate. Mouse and rat skin and liver lysates. Wild-type mouse liver lysate. Flow Cyt: 4T1 cells. IP: Mouse liver and lung whole cell lysate.

  • General notes

    ab267392 is the carrier-free version of ab253185. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free (ab267392)
    Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free (ab267392)
    All lanes : Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185) at 1/1000 dilution

    Lane 1 : Wild-type mouse liver lysate
    Lane 2 : ECM1 knockout mouse liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 63 kDa
    Observed band size: 48,85 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab253185).

    The wild-type and ECM1 knockout mouse liver lysates were kindly provided by an anonymous collaborator.

    ab253185 was shown to specifically react with Extracellular matrix protein 1 in wild-type mouse liver as signal was lost in ECM1 knockout liver. Wild-type and ECM1 knockout samples were subjected to SDS-PAGE. ab253185 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/50,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    Exposure time 7.75 secs.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free (ab267392)
    Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free (ab267392)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab253185).

    Extracellular matrix protein 1 was immunoprecipitated from 0.35 mg mouse lung lysate with ab253185 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab253185 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse lung lysate 10µg.

    Lane 2: ab253185 IP in mouse lung lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253185 in mouse lung lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

     

  • Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free (ab267392)
    Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free (ab267392)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab253185).

    Extracellular matrix protein 1 was immunoprecipitated from 0.35 mg mouse liver lysate with ab253185 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab253185 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse liver lysate 10µg.

    Lane 2: ab253185 IP in mouse liver lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253185 in mouse liver lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 mins.

     

  • Flow Cytometry - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free (ab267392)
    Flow Cytometry - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free (ab267392)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab253185).

    Flow cytometric analysis of 4% paraformaldehyde fixed 90%, methanol permeabilized 4T1 (mouse mammary gland carcinoma epithelial cell) cells labeling Extracellular matrix protein 1 with ab253185 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free (ab267392)
    Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free (ab267392)

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