Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22434-381] to Extracellular matrix protein 1
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Extracellular matrix protein 1 antibody [EPR22434-381]
    See all Extracellular matrix protein 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22434-381] to Extracellular matrix protein 1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MDA-MB-231, MDA-MB-435S and SK-MEL-28 whole cell lysates. IP: MDS-MB-435S whole cell lysate. IHC-P: Human thyroid carcinoma and carvical carcinoma tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)
    Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)

    Extracellular matrix protein 1 was immunoprecipitated from 0.35 mg of MDS-MB-435S (human mammary gland ductal carcinoma melanocyte cell line) whole cell lysate with ab253244 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab253244 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: MDS-MB-435S whole cell lysate 10μg (Input).
    Lane 2: ab253244 IP in MDA-MB-435S whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253244 in MDA-MB-435S whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

  • Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)
    Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)
    All lanes : Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244) at 1/1000 dilution

    Lane 1 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : MDA-MB-435S (human ductal carcinoma cell line) whole cell lysate
    Lane 3 : SK-MEL-28 (human malignant melanoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 61 kDa
    Observed band size: 68 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1 & 2: 3 minutes. Lane 3: 37 seconds.

    The expression molecular weight observed is consistent with what has been described in the literature (PMID: 14550953).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)

    Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labeling Extracellular matrix protein 1 with ab253244 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human cervical carcinoma is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)

    Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue labeling Extracellular matrix protein 1 with ab253244 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human thyroid carcinoma (PMID: 18374945) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)
    Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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