Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)
![Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)](https://www.abcam.com/ps/products/253/ab253244/Images/ab253244-4-253244IP1.jpg "Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22434-381] to Extracellular matrix protein 1
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Overview
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Product name
Anti-Extracellular matrix protein 1 antibody [EPR22434-381]
See all Extracellular matrix protein 1 primary antibodies -
Description
Rabbit monoclonal [EPR22434-381] to Extracellular matrix protein 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MDA-MB-231, MDA-MB-435S and SK-MEL-28 whole cell lysates. IP: MDS-MB-435S whole cell lysate. IHC-P: Human thyroid carcinoma and carvical carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22434-381 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)
Extracellular matrix protein 1 was immunoprecipitated from 0.35 mg of MDS-MB-435S (human mammary gland ductal carcinoma melanocyte cell line) whole cell lysate with ab253244 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab253244 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: MDS-MB-435S whole cell lysate 10μg (Input).
Lane 2: ab253244 IP in MDA-MB-435S whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253244 in MDA-MB-435S whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds. -
![Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)](https://www.abcam.com/ps/products/253/ab253244/Images/ab253244-3-253244WB1.jpg)
Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)All lanes : Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244) at 1/1000 dilution
Lane 1 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : MDA-MB-435S (human ductal carcinoma cell line) whole cell lysate
Lane 3 : SK-MEL-28 (human malignant melanoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 61 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lanes 1 & 2: 3 minutes. Lane 3: 37 seconds.
The expression molecular weight observed is consistent with what has been described in the literature (PMID: 14550953).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)](https://www.abcam.com/ps/products/253/ab253244/Images/ab253244-2-253244IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labeling Extracellular matrix protein 1 with ab253244 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human cervical carcinoma is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)](https://www.abcam.com/ps/products/253/ab253244/Images/ab253244-1-253244IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue labeling Extracellular matrix protein 1 with ab253244 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human thyroid carcinoma (PMID: 18374945) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)](https://www.abcam.com/ps/products/253/ab253244/Images/ab253244-5-benefits-of-recombinant-antibodies.png)
Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)
