Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] (ab208030)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20178] to hnRNP A1 (citrulline R140)
- Suitable for: WB, Dot blot
- Reacts with: Recombinant fragment
Overview
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Product name
Anti-hnRNP A1 (citrulline R140) antibody [EPR20178]
See all hnRNP A1 primary antibodies -
Description
Rabbit monoclonal [EPR20178] to hnRNP A1 (citrulline R140) -
Host species
Rabbit -
Specificity
Based on sequence homology, we predict that this antibody may react with HNRNPA1L2. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T whole cell lysate transfected with GFP tagged PADI2 or PADI4 and treated with 10mM CaCl2 for 2h, then 10uM Ionomycin for 2h.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20178 -
Isotype
IgG -
Research areas
Images
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Dot blot analysis of hnRNP A1 (citrulline R140) labeled with ab208030 at 1/1000 dilution.
Lane 1: hnRNP A1 (citrulline R140) peptide.
Lane 2: hnRNP A1 (citrulline R140) noncitrulline peptide.
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time : 3 minutes.
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All lanes : Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] (ab208030) at 1/1000 dilution
Lane 1 : Empty vector (vector control) transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HEK-293T whole cell lysate transfected with GFP tagged PADI2 and treated with 10mM CaCl2 for 2h, then 10uM Ionomycin for 2h
Lane 3 : Empty vector (vector control) transfected HEK-293T whole cell lysate
Lane 4 : HEK-293T whole cell lysate transfected with GFP tagged PADI4 and treated with 10mM CaCl2 for 2h, then 10uM Ionomycin for 2h
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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