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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] (ab208030)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] (ab208030)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20178] to hnRNP A1 (citrulline R140)
  • Suitable for: WB, Dot blot
  • Reacts with: Recombinant fragment

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Overview

  • Product name

    Anti-hnRNP A1 (citrulline R140) antibody [EPR20178]
    See all hnRNP A1 primary antibodies
  • Description

    Rabbit monoclonal [EPR20178] to hnRNP A1 (citrulline R140)
  • Host species

    Rabbit
  • Specificity

    Based on sequence homology, we predict that this antibody may react with HNRNPA1L2.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T whole cell lysate transfected with GFP tagged PADI2 or PADI4 and treated with 10mM CaCl2 for 2h, then 10uM Ionomycin for 2h.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20178
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Images

  • Dot Blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] (ab208030)
    Dot Blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] (ab208030)

    Dot blot analysis of hnRNP A1 (citrulline R140) labeled with ab208030 at 1/1000 dilution.

    Lane 1: hnRNP A1 (citrulline R140) peptide.

    Lane 2: hnRNP A1 (citrulline R140) noncitrulline peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time : 3 minutes.

  • Western blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] (ab208030)
    Western blot - Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] (ab208030)
    All lanes : Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] (ab208030) at 1/1000 dilution

    Lane 1 : Empty vector (vector control) transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : HEK-293T whole cell lysate transfected with GFP tagged PADI2 and treated with 10mM CaCl2 for 2h, then 10uM Ionomycin for 2h
    Lane 3 : Empty vector (vector control) transfected HEK-293T whole cell lysate
    Lane 4 : HEK-293T whole cell lysate transfected with GFP tagged PADI4 and treated with 10mM CaCl2 for 2h, then 10uM Ionomycin for 2h

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 39 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] (ab208030)
    Anti-hnRNP A1 (citrulline R140) antibody [EPR20178] (ab208030)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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