Anti-DEF6 antibody [OTI1E3] (ab279395)
![Anti-DEF6 antibody [OTI1E3] (ab279395)](https://www.abcam.com/ps/products/279/ab279395/Images/ab279395-2-AntiDEF6-antibody-OTI1E3-westernblot-HepG2-HeLa-SVT2-A549-COS7-Jurkat-MDCK-PC12-MCF7-cells.jpg "Anti-DEF6 antibody [OTI1E3] (ab279395)")
Key features and details
- Mouse monoclonal [OTI1E3] to DEF6
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Dog, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-DEF6 antibody [OTI1E3]
See all DEF6 primary antibodies -
Description
Mouse monoclonal [OTI1E3] to DEF6 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P HumanRecombinant fragmentWB MouseHuman
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Immunogen
Recombinant full length protein corresponding to Human DEF6 aa 1-631. NP_071330
Database link: Q9H4E7 -
Positive control
- IHC-P: Human tonsil, bladder and lymphoma tissue. WB: HepG2, HeLa, SVT2, A549, COS-7, Jurkat, MDCK, PC-12, MCF7 cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
OTI1E3 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-DEF6 antibody [OTI1E3] (ab279395)All lanes : Anti-DEF6 antibody [OTI1E3] (ab279395) at 1/2000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : SVT2 (BALB/c mouse embryonic fibroblast, SV40 transformed)
cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (Canine kidney cell line) cell extract
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (Human breast adenocarcinoma cell line) cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 74 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEF6 antibody [OTI1E3] (ab279395)](https://www.abcam.com/ps/products/279/ab279395/Images/ab279395-3-AntiDEF6-antibody-OTI1E3-immunohistochemistry-bladder-tissue-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEF6 antibody [OTI1E3] (ab279395)Paraffin embedded human bladder tissue stained for DEF6 using ab279395 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEF6 antibody [OTI1E3] (ab279395)](https://www.abcam.com/ps/products/279/ab279395/Images/ab279395-5-AntiDEF6-antibody-OTI1E3-immunohistochemistry-tonsil-tissue-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEF6 antibody [OTI1E3] (ab279395)Paraffin embedded human tonsil tissue stained for DEF6 using ab279395 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEF6 antibody [OTI1E3] (ab279395)](https://www.abcam.com/ps/products/279/ab279395/Images/ab279395-4-AntiDEF6-antibody-OTI1E3-immunohistochemistry-lymphoma-tissue-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEF6 antibody [OTI1E3] (ab279395)Paraffin embedded human lymphoma tissue stained for DEF6 using ab279395 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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![Western blot - Anti-DEF6 antibody [OTI1E3] (ab279395)](https://www.abcam.com/ps/products/279/ab279395/Images/ab279395-1-AntiDEF6-antibody-OTI1E3-westernblot-HEK293-T-cells.jpg)
Western blot - Anti-DEF6 antibody [OTI1E3] (ab279395)HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DEF6 (Right lane) cDNA for 48 hrs and lysed.
Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with ab279395 at a 1/500 dilution.