Human IL-33 ELISA Kit (ab223865)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4.7 pg/ml
- Range: 11.72 pg/ml - 750 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-33 ELISA Kit
See all IL-33 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Spiked Serum 3 2.7% Inter-assay Sample n Mean SD CV% Spiked Serum 5 3.2% -
Sample type
Cell culture supernatant, Serum, Plasma, Cell culture extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
4.7 pg/ml -
Range
11.72 pg/ml - 750 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 87 83% - 95% Cell culture extracts 95 92% - 100% Cell culture media 102 96% - 108% Hep Plasma 102 97% - 110% EDTA Plasma 106 100% - 112% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-33 ELISA Kit (ab223865) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-33 protein in cell culture supernatant, plasma, serum, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-33 with 4.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
IL-33 (Interleukin-33, also known as IL-1F11 or NF-HEV) is a proinflammatory cytokine member of the IL-1 family. It is widely expressed and functions by binding and signaling through the IL-1RL1/ST2 receptor. IL-33 is expressed as a pro-protein; the antibodies in this kit were generated using the mature form of IL-33 (aa 109-270).
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml 10X Human IL-33 Capture Antibody 1 x 600µl 10X Human IL-33 Detector Antibody 1 x 600µl Human IL-33 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).
In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or proinflammatory activation (PubMed:18787100). -
Tissue specificity
Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta. -
Sequence similarities
Belongs to the IL-1 family. Highly divergent. -
Domain
The homeodomain-like HTH domain mediates nuclear localization and heterochromatin association. -
Post-translational
modificationsThe full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase. -
Cellular localization
Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes (PubMed:17185418). - Information by UniProt
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Alternative names
- C9orf26
- CHROMOSOME 9 OPEN READING FRAME 26
- DKFZp586H0523
see all -
Database links
- Entrez Gene: 90865 Human
- Omim: 608678 Human
- SwissProt: O95760 Human
- Unigene: 731660 Human
Images
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Other - Human IL-33 ELISA Kit (ab223865)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human IL33 standard curve in Sample Diluent 50BP.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human IL33 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human IL33 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spike IL33 in human serum, plasma and cell culture media (RPMI + 10% FBS) samples.The concentrations of IL33 were measured in duplicates, interpolated from the IL33 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum neat, plasma (heparin) neat, plasma (EDTA) neat and RPMI + 10% FBS neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native IL33 in human PMA/LPS stimulated THP-1 cell extract based on a 1,000 µg/mL extract load.The concentrations of IL33 were measured in duplicate and interpolated from the IL33 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL33 concentration was determined to be 351.5 pg/mL in PMA/LPS stimulated THP-1 cell extract and undetectable in unstimulated THP-1 cell extract (not shown).
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Comparison of IL33 in unstimulated and PMA/LPS stimulated THP-1 cell supernatants.THP-1 cells were cultured for 24 hours in the presence or absence of 50 ng/mL PMA and 10 µg/mL LPS. The concentrations of IL33 were measured in neat supernatant samples in quadruplicates and interpolated from the IL33 standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean IL33 concentration was determined to be 15.95 pg/mL in neat PMA/LPS stimulated THP-1 cell culture supernatant and undetectable in neat unstimulated THP-1 cell culture supernatant and RPMI media (not shown).
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Sandwich ELISA - Human IL-33 ELISA Kit (ab223865)To learn more about the advantages of recombinant antibodies see here.
