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Human IL-33 ELISA Kit (ab119547)

Key features and details

  • Sensitivity: 0.2 pg/ml
  • Range: 7.8 pg/ml - 500 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human IL-33 ELISA Kit
    See all IL-33 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 6.9%

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.2 pg/ml

  • Range

    7.8 pg/ml - 500 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 73% - 92%
    Serum 67% - 79%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s IL-33 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-33 in cell culture supernatant, serum, and plasma (citrate, heparin).


    IL-33 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Human IL-33 detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-33 captured on the plate.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 6 units
    Biotin-Conjugate anti-human IL-33 polyclonal antibody 1 x 140µl
    Calibrator Diluent 1 x 5ml
    Conjugate Diluent 1 x 15ml
    IL-33 Standard lyophilized (500 pg/mL upon reconstitution) 2 vials
    Microplate coated with polyclonal antibody to IL-33 (12 x 8 wells) 1 unit
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml

  • Research areas

  • Function

    Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).
    In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or proinflammatory activation (PubMed:18787100).

  • Tissue specificity

    Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.

  • Sequence similarities

    Belongs to the IL-1 family. Highly divergent.

  • Domain

    The homeodomain-like HTH domain mediates nuclear localization and heterochromatin association.

  • Post-translational
    modifications

    The full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase.

  • Cellular localization

    Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes (PubMed:17185418).
  • Information by UniProt

  • Alternative names

    • C9orf26
    • CHROMOSOME 9 OPEN READING FRAME 26
    • DKFZp586H0523
    • DVS27
    • DVS27 related protein
    • IL 1F11
    • IL 33
    • IL-1F11
    • IL-33
    • IL1F11
    • IL33
    • IL33_HUMAN
    • Interleukin 1 family member 11
    • Interleukin 33
    • INTERLEUKIN 33 NFHEV
    • Interleukin 33 precursor
    • Interleukin-1 family member 11
    • Interleukin-33 (109-270)
    • Interleukin33
    • NF HEV
    • NF-HEV
    • NFEHEV
    • NFHEV
    • Nuclear factor for high endothelial venules
    • Nuclear factor from high endothelial venules
    • OTTHUMP00000021041
    • RP11 575C20.2
    see all

  • Database links

    Images

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab119547.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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