Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [1/MCM6] to MCM6 - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-MCM6 antibody [1/MCM6] - BSA and Azide free
    See all MCM6 primary antibodies

  • Description

    Mouse monoclonal [1/MCM6] to MCM6 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • WB: MCF7, HeLa, SW480, U-2 OS and A431, Human tonsil lysates. IHC-P: Human colon and tonsil tissues. ICC: A431, U-2 OS cells.

  • General notes

    ab280215 is the carrier-free version of ab280208. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280215 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)
    Western blot - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)
    All lanes : Anti-MCM6 antibody [1/MCM6] (ab280208) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate
    Lane 3 : SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 4 : U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate
    Lane 5 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
    Lane 6 : Human tonsil tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 93 kDa



    This data was developed using ab280208 the same antibody clone in a different buffer formulation.

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST

    Exposure time lane 1-5: 37 seconds
    lane 6: 3 minutes

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)

    This data was developed using ab280208 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MCM6 with ab280208 at 1/200 (4.945 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining in human colon.The section was incubated with ab280208 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)

    This data was developed using ab280208 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MCM6 with ab280208 at 1/200 (4.945 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining in human tonsil.The section was incubated with ab280208 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunocytochemistry - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)
    Immunocytochemistry - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)

    This data was developed using ab280208 the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 cells labelling MCM6 with ab280208 at 1/20 (49.45 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in A431 cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunocytochemistry - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)
    Immunocytochemistry - Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)

    This data was developed using ab280208 the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-2 OS cells labelling MCM6 with ab280208 at 1/20 (49.45 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in U-2 OS cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)
    Anti-MCM6 antibody [1/MCM6] - BSA and Azide free (ab280215)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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