Mouse NT-ProANP ELISA Kit
(ab267800)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 43.4 pg/ml
- Range: 125 pg/ml - 8000 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, EDTA Plasma, Hep Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
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Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 8 3.6% Inter-assay Sample n Mean SD CV% Plasma 3 4.9% -
Sample type
Cell culture supernatant, Cell culture media, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
43.4 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 99 84% - 111% Cell culture media 110 99% - 122% Hep Plasma 92 81% - 104% EDTA Plasma 88 80% - 97% Cit plasma 100 85% - 117% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse NT-ProANP ELISA Kit (ab267800) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NT-ProANP protein in cell culture supernatant, edta plasma, hep plasma, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse NT-ProANP with 43.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Mouse NT-ProANP is the propeptide portion of the 129 amino acids long Atrial Natriuretic Peptide (ANP) preproprotein. ANP is primarily synthesized in the heart and is secreted in response to atrial distention. The ANP proprotein is cleaved by serine protease corin to produce the functional hormone, atrial natriuretic factor (ANF). ANF circulates in plasma; it is involved in cardiovascular homeostasis through the regulation of natriuresis, diuresis, and vasodilation directed toward reduction of body fluid and blood pressure regulation. NT-ProANP circulates at low levels at the early stages of hypertension and increases during chronic heart failure. Mouse NT-ProANP is 94% identical to rat NT-ProANP on amino acid level. This kit recognizes mouse NT-ProANP protein (amino acids 25 – 122 of Uniprot # P05125). This kit does not recognize mouse ANP proprotein (amino acids 25 – 152 of Uniprot # P05125).
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse NT-ProANP Capture Antibody 1 x 600µl 10X Mouse NT-ProANP Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Mouse NT-ProANP Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. -
Involvement in disease
Atrial standstill 2
Atrial fibrillation, familial, 6 -
Sequence similarities
Belongs to the natriuretic peptide family. -
Post-translational
modificationsCleaved by CORIN upon secretion to produce the functional hormone.
Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ANF
- ANF_HUMAN
- ANP
see all -
Database links
- Entrez Gene: 230899 Mouse
- SwissProt: P05125 Mouse
- Unigene: 482123 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse NT-ProANP standard curve in Sample Diluent NS.The NT-ProANP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native NT-ProANP in mouse plasma samples.The concentrations of NT-ProANP were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 5%, plasma (heparin) 5%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 103.6 ng/mL in neat plasma (citrate), 50.31 ng/mL in neat plasma (heparin), and 28.27 ng/mL in neat plasma (EDTA).
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Interpolated concentrations of native NT-ProANP in mouse heart tissue culture supernatant samples.The concentrations of NT-ProANP were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: mouse heart tissue culture supernatant 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 642.6 ng/mL in neat mouse heart tissue culture supernatant. No detectable signal was seen in mouse brain tissue culture supernatant samples (not shown).
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Sandwich ELISA - Mouse NT-ProANP ELISA Kit (ab267800)To learn more about the advantages of recombinant antibodies see here.
