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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-Fyn (phospho Y530) + Yes (phospho Y537) antibody [EPR13512] (ab188319)

Price and availability

278 083 ₸

Availability

Order now and get it on Friday March 05, 2021

Anti-Fyn (phospho Y530) + Yes (phospho Y537) antibody [EPR13512] (ab188319)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
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  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13512] to Fyn (phospho Y530) + Yes (phospho Y537)
  • Suitable for: WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-Fyn (phospho Y530) + Yes (phospho Y537) antibody [EPR13512]
    See all Fyn (phospho Y530) + Yes (phospho Y537) primary antibodies
  • Description

    Rabbit monoclonal [EPR13512] to Fyn (phospho Y530) + Yes (phospho Y537)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Lysate from HeLa cells treated with pervanadate; IP: HeLa cells treated with pervanadate.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13512
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Src Family
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Tyrosine kinases
    • Src family

Images

  • Western blot - Anti-Fyn (phospho Y530) + Yes (phospho Y537) antibody [EPR13512] (ab188319)
    Western blot - Anti-Fyn (phospho Y530) + Yes (phospho Y537) antibody [EPR13512] (ab188319)
    All lanes : Anti-Fyn (phospho Y530) + Yes (phospho Y537) antibody [EPR13512] (ab188319) at 1/10000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50 nM pervanadate for 1 hour whole cell lysates
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50 nM pervanadate for 1 hour whole cell lysates. Then the membrane was incubated with phosphatase

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 61 kDa
    Observed band size: 59 kDa
    why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-Fyn (phospho Y530) + Yes (phospho Y537) antibody [EPR13512] (ab188319)
    Immunoprecipitation - Anti-Fyn (phospho Y530) + Yes (phospho Y537) antibody [EPR13512] (ab188319)

    ab188319 (purified) at 1:30 dilution (2µg) immunoprecipitating Fyn (phospho Y530) + Yes (phospho Y537) in HeLa treated with 50nM Pervandate for 1h whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM Pervandate for 1h whole cell lysate 10µg
    Lane 2 (+): ab188319 & HeLa treated with 50nM Pervandate for 1h whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab188319 in HeLa treated with 50nM Pervandate for 1h whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-Fyn (phospho Y530) + Yes (phospho Y537) antibody [EPR13512] (ab188319)
    Western blot - Anti-Fyn (phospho Y530) + Yes (phospho Y537) antibody [EPR13512] (ab188319)
    All lanes : Anti-Fyn (phospho Y530) + Yes (phospho Y537) antibody [EPR13512] (ab188319) at 1/20000 dilution (Unpurified)

    Lane 1 : Lysate from HeLa cells treated with pervanadate
    Lane 2 : Lysate from untreated HeLa cells

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 61 kDa
    Observed band size: 59 kDa why is the actual band size different from the predicted?

  • Anti-Fyn (phospho Y530) + Yes (phospho Y537) antibody [EPR13512] (ab188319)
    Anti-Fyn (phospho Y530) + Yes (phospho Y537) antibody [EPR13512] (ab188319)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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