Mouse IGFBP1 ELISA Kit (ab272465)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 15.55 pg/ml
- Range: 34.38 pg/ml - 2200 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IGFBP1 ELISA Kit
See all IGFBP1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.5% Inter-assay Sample n Mean SD CV% Serum 3 4.6% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
15.55 pg/ml -
Range
34.38 pg/ml - 2200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 99 92% - 107% Serum 111 105% - 119% Hep Plasma 110 94% - 122% EDTA Plasma 103 99% - 108% Cit plasma 99 97% - 101% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse IGFBP1 ELISA kit (ab272465) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse IGFBP1 protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IGFBP1 with 15.55 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Insulin-like growth factor-binding protein 1 (IGFBP1) is an IGF binding protein involved in prolonging its half-life and has both inhibitory and stimulatory effects on IGFs. IGFBP1 can also alter the interaction of IGFs with their receptors. IGFBP1 is additionally involved in cell migration.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse IGFBP1 Capture Antibody 1 x 600µl 10X Mouse IGFBP1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse IGFBP1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. -
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
Post-translational
modificationsPhosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- AFBP
- Alpha pregnancy associated endometrial globulin
- Amniotic fluid binding protein
see all -
Database links
- Entrez Gene: 16006 Mouse
- SwissProt: P47876 Mouse
- Unigene: 21300 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse IGFBP1 standard curve in Sample Diluent NS.The IGFBP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IGFBP1 in mouse serum, and plasma samples. -
Interpolated concentrations of native IGFBP1 in mouse liver tissue supernatant samples.The concentrations of IGFBP1 were measured in duplicates, interpolated from the IGFBP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: liver supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP1 concentration was determined to be 4.69 ng/mL in liver supernatant.
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Sandwich ELISA - Mouse IGFBP1 ELISA Kit (ab272465)To learn more about the advantages of recombinant antibodies see here.
