Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IGFBP4 antibody [EPR19762] (ab205581)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19762] to IGFBP4
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-IGFBP4 antibody [EPR19762]
    See all IGFBP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR19762] to IGFBP4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549 and HT-29 whole cell lysates; Human placenta lysate. Flow Cyt: A549 cells. IP: A549 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IGFBP4 antibody [EPR19762] (ab205581)
    Western blot - Anti-IGFBP4 antibody [EPR19762] (ab205581)
    Anti-IGFBP4 antibody [EPR19762] (ab205581) at 1/1000 dilution + A549 (human lung carcinoma cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 28 kDa
    Observed band size: 18,28 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human IGFBP4 has 2 isoforms detected at 28 kDa and 18 kDa.

  • Western blot - Anti-IGFBP4 antibody [EPR19762] (ab205581)
    Western blot - Anti-IGFBP4 antibody [EPR19762] (ab205581)
    Anti-IGFBP4 antibody [EPR19762] (ab205581) at 1/1000 dilution + human placenta lysate at 10 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-IGFBP4 antibody [EPR19762] (ab205581)
    Western blot - Anti-IGFBP4 antibody [EPR19762] (ab205581)
    Anti-IGFBP4 antibody [EPR19762] (ab205581) at 1/1000 dilution + HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-IGFBP4 antibody [EPR19762] (ab205581)
    Flow Cytometry - Anti-IGFBP4 antibody [EPR19762] (ab205581)

    Flow cytometric analysis of 4% paraformaldehyde-fixed A549 (human lung carcinoma cell line) cell line labeling IGFBP4 with ab205581 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-IGFBP4 antibody [EPR19762] (ab205581)
    Immunoprecipitation - Anti-IGFBP4 antibody [EPR19762] (ab205581)

    IGFBP4 was immunoprecipitated from 0.35 mg of A549 (human lung carcinoma cell line) whole cell lysate with ab205581 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab205581 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: A549 whole cell lysate 10 µg (Input). 

    Lane 2: ab205581 IP in A549 whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205581 in A549 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    Note: Human IGFBP4 has 2 isoforms detected at 28 kDa and 18 kDa.

  • Anti-IGFBP4 antibody [EPR19762] (ab205581)
    Anti-IGFBP4 antibody [EPR19762] (ab205581)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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