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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-ADCK4 antibody (ab223114)

Anti-ADCK4 antibody (ab223114)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to ADCK4
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ADCK4 antibody
    See all ADCK4 primary antibodies
  • Description

    Rabbit polyclonal to ADCK4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human ADCK4 aa 10-137.
    Database link: Q96D53

  • Positive control

    • WB: HeLa, A549 and HepG2 whole cell lysate. IHC-P: Human kidney tissue and melanoma cancer tissues. ICC/IF: A549 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Images

  • Western blot - Anti-ADCK4 antibody (ab223114)
    Western blot - Anti-ADCK4 antibody (ab223114)
    All lanes : Anti-ADCK4 antibody (ab223114) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 60 kDa
    Observed band size: 56,60 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-ADCK4 antibody (ab223114)
    Immunocytochemistry/ Immunofluorescence - Anti-ADCK4 antibody (ab223114)

    A549 (human lung carcinoma cell line) cells stained for ADCK4 (green) using ab223114 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADCK4 antibody (ab223114)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADCK4 antibody (ab223114)

    Paraffin-embedded human melanoma cancer tissue stained for ADCK4 using ab223114 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADCK4 antibody (ab223114)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADCK4 antibody (ab223114)

    Paraffin-embedded human kidney tissue stained for ADCK4 using ab223114 at 1/100 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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