Human PNP ELISA Kit (Purine Nucleoside Phosphorylase) (ab260069)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 40 pg/ml
- Range: 78.13 pg/ml - 5000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PNP ELISA Kit (Purine Nucleoside Phosphorylase)
See all Nucleoside phosphorylase kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 8.3% Inter-assay Sample n Mean SD CV% Serum 3 4.1% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
40 pg/ml -
Range
78.13 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 96 85% - 108% Hep Plasma 90 82% - 104% EDTA Plasma 96 85% - 110% Cit plasma 103 90% - 114% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human PNP ELISA Kit (Purine Nucleoside Phosphorylase) (ab260069) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PNP (Purine Nucleoside Phosphorylase) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PNP (Purine Nucleoside Phosphorylase) with 40 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Purine Nucleoside Phosphorylase (PNP) is a catabolic enzyme which breaks down purine residues by releasing the nucleobase and phosphorylating the ribose residue. It is also used to produce nucleotide monobases as a salvage pathway. PNP plays an important role in the maintenance of T-cells. This kit targets the full-length protein.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human PNP (Purine Nucleoside Phosphorylase) Capture Antibody 1 x 600µl 10X Human PNP (Purine Nucleoside Phosphorylase) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Human PNP (Purine Nucleoside Phosphorylase) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Involvement in disease
Defects in PNP are the cause of purine nucleoside phosphorylase deficiency (PNP deficiency) [MIM:613179]. It leads to a severe T-cell immunodeficiency with neurologic disorder in children. -
Sequence similarities
Belongs to the PNP/MTAP phosphorylase family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Alternative names
- FLJ94043
- FLJ97288
- FLJ97312
see all -
Database links
- Entrez Gene: 4860 Human
- Omim: 164050 Human
- SwissProt: P00491 Human
- Unigene: 75514 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human PNP standard curve in Sample Diluent NS.The PNP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native PNP in human serum and plasma samples.The concentrations of PNP were measured in duplicate, interpolated from the PNP standard curves, and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (heparin) 100% and plasma (edta) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PNP concentration was determined to be 1435.64 pg/mL in serum, 4419.64 pg/mL in plasma (citrate), 2483.57 pg/mL in plasma (heparin), and 1750.71 pg/mL in plasma (edta).
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Sandwich ELISA - Human PNP ELISA Kit (Purine Nucleoside Phosphorylase) (ab260069)To learn more about the advantages of recombinant antibodies see here.
