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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-IGFBP4 antibody [A3] (ab239512)

Anti-IGFBP4 antibody [A3] (ab239512)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [A3] to IGFBP4
  • Suitable for: IHC-P, WB
  • Reacts with: Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-IGFBP4 antibody [A3]
    See all IGFBP4 primary antibodies
  • Description

    Mouse monoclonal [A3] to IGFBP4
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein (His-tag) corresponding to Human IGFBP4 aa 1 to the C-terminus. N-terminal tag (Expressed in E.coli).
    Database link: P22692-1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human liver, prostate gland cancer, glioma and stomach tissues. WB: Recombinant human IGFBP4 protein; Rat placenta and uterus lysates.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS.
  • Clonality

    Monoclonal
  • Clone number

    A3
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • Other
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Other
    • Signal Transduction
    • Growth Factors/Hormones
    • Insulin / Insulin-like
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone

Images

  • Western blot - Anti-IGFBP4 antibody [A3] (ab239512)
    Western blot - Anti-IGFBP4 antibody [A3] (ab239512)
    Anti-IGFBP4 antibody [A3] (ab239512) at 2 µg/ml + Rat placenta lysate

    Secondary
    HRP-Goat Anti-Mouse IgG at 1/5000 dilution

    Predicted band size: 28 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP4 antibody [A3] (ab239512)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP4 antibody [A3] (ab239512)

    Paraffin-embedded human stomach tissue stained for IGFBP4 with ab239512 at 10 µg/ml in immunohistochemical analysis. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP4 antibody [A3] (ab239512)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP4 antibody [A3] (ab239512)

    Paraffin-embedded human glioma tissue stained for IGFBP4 with ab239512 at 30 µg/ml in immunohistochemical analysis. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP4 antibody [A3] (ab239512)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP4 antibody [A3] (ab239512)

    Paraffin-embedded human prostate gland cancer tissue stained for IGFBP4 with ab239512 at 30 µg/ml in immunohistochemical analysis. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP4 antibody [A3] (ab239512)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP4 antibody [A3] (ab239512)

    Paraffin-embedded human liver tissue stained for IGFBP4 with ab239512 at 30 µg/ml in immunohistochemical analysis. DAB staining.

  • Western blot - Anti-IGFBP4 antibody [A3] (ab239512)
    Western blot - Anti-IGFBP4 antibody [A3] (ab239512)
    Anti-IGFBP4 antibody [A3] (ab239512) at 2 µg/ml + Rat uterus lysate

    Predicted band size: 28 kDa

  • Western blot - Anti-IGFBP4 antibody [A3] (ab239512)
    Western blot - Anti-IGFBP4 antibody [A3] (ab239512)
    Anti-IGFBP4 antibody [A3] (ab239512) at 5 µg/ml + Recombinant human IGFBP4 protein

    Secondary
    HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution

    Predicted band size: 28 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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