Anti-AS160 (phospho T642) antibody (ab65753)
Key features and details
- Sheep polyclonal to AS160 (phospho T642)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AS160 (phospho T642) antibody
See all AS160 primary antibodies -
Description
Sheep polyclonal to AS160 (phospho T642) -
Host species
Sheep -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human AS160 aa 637-647 conjugated to keyhole limpet haemocyanin.
Sequence:CRRRAHTFSHPP
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Positive control
- WB: HEK-293 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Preservative: 0.05% Sodium azide
Constituents: 0.238% HEPES, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AS160 (phospho T642) antibody (ab65753)All lanes : Anti-AS160 (phospho T642) antibody (ab65753) at 2 µg/ml
Lane 1 : AS160 wild type (WT)
Lane 2 : AS160 wild type (WT) with (+) 1 µM insulin treatment for 15 minutes
Lane 3 : S588A mutant (S588A)
Lane 4 : S588A mutant (S588A) with (+) 1 µM insulin treatment for 15 minutes
Lane 5 : T642A mutant (T642A)
Lane 6 : T642A mutant (T642A) with (+) 1 µM insulin treatment for 15 minutes
Predicted band size: 147 kDaWestern blot analysis of immunoprecipitated Flagtagged AS160 wild type (WT), S588A mutant (S588A), and T642A mutant (T642A) over-expressed transiently in HEK-293 cells, basal (-) or with (+) 1 µM insulin treatment for 15 minutes.
