Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-AS160 antibody [EPR18418] (ab189890)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18418] to AS160
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-AS160 antibody [EPR18418]
See all AS160 primary antibodies
Description

Rabbit monoclonal [EPR18418] to AS160
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal heart and fetal kidney lysates; A-673, MCF7, HeLa, HEK-293, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse and rat brain lysates. IP: HEK-293 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18418
Isotype

IgG
Research areas

Western blot - Anti-AS160 antibody [EPR18418] (ab189890)

All lanes : Anti-AS160 antibody [EPR18418] (ab189890) at 1/1000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution

Predicted band size: 146 kDa
Observed band size: 160 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 30 seconds; Lane 2: 5 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID: 19470471).
Western blot - Anti-AS160 antibody [EPR18418] (ab189890)

All lanes : Anti-AS160 antibody [EPR18418] (ab189890) at 1/5000 dilution

Lane 1 : A-673 (Human muscle Ewing's Sarcoma cell line) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 146 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 3 minutes; Lane 2: 1 minute; Lane 3: 10 seconds; Lane 4: 5 seconds.
Western blot - Anti-AS160 antibody [EPR18418] (ab189890)

All lanes : Anti-AS160 antibody [EPR18418] (ab189890) at 1/1000 dilution

Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 4 : Mouse brain lysate
Lane 5 : Rat brain lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 146 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 1 minute; Lane 2: 3 minutes; Lane 3: 30 seconds; Lane 4: 30 seconds; Lane 5: 3 minutes.
Immunoprecipitation - Anti-AS160 antibody [EPR18418] (ab189890)

AS160 was immunoprecipitated from 0.35 mg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate with ab189890 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab189890 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: HEK-293 whole cell lysate 10 μg (Input).

Lane 2: ab189890 IP in HEK-293 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab189890 in HEK-293 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds.
Anti-AS160 antibody [EPR18418] (ab189890)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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