Anti-Vaspin antibody [EPR23079-196] (ab267470)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23079-196] to Vaspin
- Suitable for: WB, IP
- Reacts with: Mouse
Overview
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Product name
Anti-Vaspin antibody [EPR23079-196]
See all Vaspin primary antibodies -
Description
Rabbit monoclonal [EPR23079-196] to Vaspin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse skin and stomach tissue lysates; His-tagged recombinant mouse Vaspin protein (aa 20-413). IP: Mouse stomach tissue lysate.
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General notes
This antibody (Clone EPR23079-196) is a different clone to ab267471 (Clone EPR23080-232).
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23079-196 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Vaspin antibody [EPR23079-196] (ab267470) at 1/1000 dilution
Lane 1 : Mouse skin tissue lysate
Lane 2 : Mouse stomach tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 19554505).
Negative control: Mouse kidney (PMID: 19554505).
Exposure time: 26 seconds.
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Vaspin was immunoprecipitated from 0.35 mg Mouse stomach tissue lysate 10 with ab267470 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267470 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.
Lane 1: Mouse stomach tissue lysate 10 ug
Lane 2: ab267470 IP in Mouse stomach tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267470 in mouse stomach tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds
Vaspin protein can form polymers when heated as described in the literature (PMID: 28668641).
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Anti-Vaspin antibody [EPR23079-196] (ab267470) at 1/1000 dilution + His-tagged recombinant mouse Vaspin protein (aa 20-413), 20 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST Exposure time: 26 seconds
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