Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD30 antibody [BLR055F] - BSA free (ab272089)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR055F] to CD30 - BSA free
  • Suitable for: WB, IP, IHC-P, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-CD30 antibody [BLR055F] - BSA free
    See all CD30 primary antibodies

  • Description

    Rabbit monoclonal [BLR055F] to CD30 - BSA free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human CD30 aa 1-400. NP_001234.3. Extracellular domain.
    Database link: P28908

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IP: MJ whole cell lysate. IHC-P: Human tonsil tissue. ICC: SR cells.

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

    ab272089 is the carrier-free version of ab252561. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Properties

Images

  • Immunocytochemistry - Anti-CD30 antibody [BLR055F] - BSA free (ab272089)
    Immunocytochemistry - Anti-CD30 antibody [BLR055F] - BSA free (ab272089)

    Formalin-fixed, paraffin-embedded sections of SR (human large cell immunoblastic lymphoma cell line) cells stained for CD30 using ab252561 at 1/250 dilution in immunohistochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: DAB staining.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252561).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [BLR055F] - BSA free (ab272089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [BLR055F] - BSA free (ab272089)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD30 using ab252561 at 1/250 dilution in immunohistochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: DAB staining.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252561).

  • Immunoprecipitation - Anti-CD30 antibody [BLR055F] - BSA free (ab272089)
    Immunoprecipitation - Anti-CD30 antibody [BLR055F] - BSA free (ab272089)

    CD30 was immunoprecipitated from MJ (human cutaneous T cell lymphoma cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab252561 at 5 µL per reaction. Western blot was performed from the immunoprecipitate using ab252561 at 1/1000 dilution.

    Lane 1: ab252561 IP in MJ whole cell lysate.
    Lane 2: Control IgG IP in MJ whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

    Lysates prepared in NETN buffer.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252561).

  • Anti-CD30 antibody [BLR055F] - BSA free (ab272089)
    Anti-CD30 antibody [BLR055F] - BSA free (ab272089)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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