Anti-NDUFA8 antibody [EPR15303] (ab184952)
![Anti-NDUFA8 antibody [EPR15303] (ab184952)](https://www.abcam.com/ps/products/184/ab184952/Images/ab184952-217636-anti-ndufa8-antibody-epr15303-western-blot.jpg "Anti-NDUFA8 antibody [EPR15303] (ab184952)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15303] to NDUFA8
- Suitable for: IP, WB
- Reacts with: Human
Overview
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Product name
Anti-NDUFA8 antibody [EPR15303]
See all NDUFA8 primary antibodies -
Description
Rabbit monoclonal [EPR15303] to NDUFA8 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal heart, Human fetal muscle, MCF7 and HepG2 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15303 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NDUFA8 antibody [EPR15303] (ab184952)All lanes : Anti-NDUFA8 antibody [EPR15303] (ab184952) at 1/20000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal muscle lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
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![Immunoprecipitation - Anti-NDUFA8 antibody [EPR15303] (ab184952)](https://www.abcam.com/ps/products/184/ab184952/Images/ab184952-217639-anti-ndufa8-antibody-epr15303-immunoprecipitation.jpg)
Immunoprecipitation - Anti-NDUFA8 antibody [EPR15303] (ab184952)Western blot analysis of NDUFA8 in Human fetal heart lysate immunoprecipitated with ab184952 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Human fetal heart lysate.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
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![Anti-NDUFA8 antibody [EPR15303] (ab184952)](https://www.abcam.com/ps/products/184/ab184952/Images/ab184952-3-benefits-of-recombinant-antibodies.png)
Anti-NDUFA8 antibody [EPR15303] (ab184952)
