Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Corin antibody [EPR23361-77] (ab274320)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23361-77] to Corin
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Corin antibody [EPR23361-77]
    See all Corin primary antibodies

  • Description

    Rabbit monoclonal [EPR23361-77] to Corin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human and mouse heart tissue lysate. IHC-P: Human cardiac muscle and kidney tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR23361-77] (ab274320)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR23361-77] (ab274320)

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Corin with ab274320 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cardiac muscle (PMID: 11082206). The section was incubated with ab274320 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Corin antibody [EPR23361-77] (ab274320)
    Western blot - Anti-Corin antibody [EPR23361-77] (ab274320)
    Anti-Corin antibody [EPR23361-77] (ab274320) at 1/1000 dilution + Mouse heart tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 116 kDa
    Observed band size: 100-180 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Tissue specific protein, highly expressed in heart (PMID: 11082006).

    The expression profile observed is consistent with what has been described in the literature (PMID: 29387201, 17996891).

    Exposure time: 10 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR23361-77] (ab274320)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR23361-77] (ab274320)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Corin with ab274320 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human kidney. The section was incubated with ab274320 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Corin antibody [EPR23361-77] (ab274320)
    Western blot - Anti-Corin antibody [EPR23361-77] (ab274320)
    All lanes : Anti-Corin antibody [EPR23361-77] (ab274320) at 1/1000 dilution

    Lane 1 : Human heart tissue lysate
    Lane 2 : Human brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 116 kDa
    Observed band size: 100-180 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Tissue specific protein, highly expressed in heart (PMID: 11082006).

    The expression profile observed is consistent with what has been described in the literature (PMID: 29387201, 17996891).

    Low expression: human brain (PMID: 11082006).

    Exposure time: 3 minutes.

     

  • Anti-Corin antibody [EPR23361-77] (ab274320)
    Anti-Corin antibody [EPR23361-77] (ab274320)

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