Anti-AS160 antibody (ab134749)
Key features and details
- Rabbit polyclonal to AS160
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AS160 antibody
See all AS160 primary antibodies -
Description
Rabbit polyclonal to AS160 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human AS160 aa 1248-1298 (C terminal).
Sequence:IRT LEQEKMAYQK TVEQLRKLLP ADALVNCDLL LRDLNCNPNN KAKIGNKP
Database link: NP_055647.2 -
Positive control
- Whole cell lysate from HeLa, 293T and Jurkat cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7.0 to 8.0 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AS160 antibody (ab134749)All lanes : Anti-AS160 antibody (ab134749) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from 293T cells at 50 µg
Lane 4 : Whole cell lysate from Jurkat cells at 50 µg
Developed using the ECL technique.
Predicted band size: 146 kDa
Exposure time: 10 seconds
Western Blot Gel 4-8% -
Immunoprecipitation - Anti-AS160 antibody (ab134749)Lane 1: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an upstream epitope of AS160.
Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an upstream epitope of AS160.
Lane 3: Detection of Human AS160 by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab134749 at 6 µg/mg lysate for IP.
Lane 4: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab134749 at 1 µg/ml.
Western Blot Gel 4-8%
Developed using the ECL technique with an exposure time of 3 seconds.
