Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2733(2)] to AS160 (phospho T642)
  • Suitable for: Dot blot, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-AS160 (phospho T642) antibody [EPR2733(2)]
    See all AS160 primary antibodies

  • Description

    Rabbit monoclonal [EPR2733(2)] to AS160 (phospho T642)

  • Host species

    Rabbit

  • Specificity

    ab131214 only detects AS160 when phosphorylated at Threonine 642.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human AS160 (phospho T642). The exact sequence is proprietary.
    Database link: O60343

  • Positive control

    • WB: HEK-293 grown in serum free media overnight, then treated with 100nM Calyculin A (ab141784) for 50min and then 100ng/ml Insulin was added for the last 20min, whole cell lysate and 293T cell lysate - insulin-treated. Dot Blot: AS160 (phospho T642) phospho peptide.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 6.40 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR2733(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)
    Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)
    All lanes : Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214) at 1.12 µg/ml

    Lane 1 : HEK-293 (human embryonic kidney epithelial cell) grown in serum free media overnight whole cell lysate
    Lane 2 : HEK-293 grown in serum free media overnight, then treated with 100nM Calyculin A (ab141784) for 50min and then 100ng/ml Insulin was added for the last 20min, whole cell lysate
    Lane 3 : HEK-293 grown in serum free media overnight, then treated with 100nM Calyculin A (ab141784) for 50min and then 100ng/ml Insulin was added for the last 20min, whole cell lysate. Then the membrane was incubated with alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 146 kDa



    Blocking and diluting buffer: 5% NFDM/TBST. 

  • Dot Blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)
    Dot Blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)

    Dot blot analysis of AS160 (phospho T642) phospho peptide (Lane 1) and AS160 non-phospho peptide (Lane 2) labelling AS160 (phospho T642) phospho peptide with ab131214 at a dilution of 1:1000 dilution (1.12μg/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1:20,000 dilution.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)
    Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)
    All lanes : Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214) at 1/1000 dilution

    Lane 1 : 293T cell lysate - untreated
    Lane 2 : 293T cell lysate - insulin-treated

    Predicted band size: 146 kDa



    Bottom pannel shows detection of total AS160 with a general anti AS160 antibody.

  • OI-RD Scanning - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)
    OI-RD Scanning - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)
    Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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