Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKD2 antibody [EP1495Y] - BSA and Azide free (ab247314)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1495Y] to PKD2 - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PKD2 antibody [EP1495Y] - BSA and Azide free
    See all PKD2 primary antibodies

  • Description

    Rabbit monoclonal [EP1495Y] to PKD2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247314 is the carrier-free version of ab51250.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

Images

  • Western blot - Anti-PKD2 antibody [EP1495Y] - BSA and Azide free (ab247314)
    Western blot - Anti-PKD2 antibody [EP1495Y] - BSA and Azide free (ab247314)

    This data was developed using ab51250, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (40 µg)

    Lane 2: PKD2 knockout HAP1 cell lysate (40 µg)

    Lane 3: HeLa cell lysate (20 µg)

    Lane 4: HEK293 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab51250 observed at 110 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab51250 was shown to recognize PKD2 when PKD2 knockout samples were used, along with additional cross-reactive bands. Wild-type and PKD2 knockout samples were subjected to SDS-PAGE. ab51250 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1:10,000 dilution respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PKD2 antibody [EP1495Y] - BSA and Azide free (ab247314)
    Western blot - Anti-PKD2 antibody [EP1495Y] - BSA and Azide free (ab247314)
    Anti-PKD2 antibody [EP1495Y] (ab51250) at 1/500 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 97 kDa
    Observed band size: 105 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab51250, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKD2 antibody [EP1495Y] - BSA and Azide free (ab247314)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKD2 antibody [EP1495Y] - BSA and Azide free (ab247314)
    This data was developed using ab51250, the same antibody clone in a different buffer formulation.Ab51250 (1:100) staining human PKD2 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-PKD2 antibody [EP1495Y] - BSA and Azide free (ab247314)
    Immunocytochemistry - Anti-PKD2 antibody [EP1495Y] - BSA and Azide free (ab247314)
    This data was developed using ab51250, the same antibody clone in a different buffer formulation.ICC/IF image of ab51250 stained HeLa cells. The cells were 4% Formaldehyde fixed (10 mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51250, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Anti-PKD2 antibody [EP1495Y] - BSA and Azide free (ab247314)
    Anti-PKD2 antibody [EP1495Y] - BSA and Azide free (ab247314)

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