Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] (ab223500)
![Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] (ab223500)](https://www.abcam.com/ps/products/223/ab223500/Images/ab223500-283000-anti-erk1-erk2-phospho-t202-y204-antibody-sp327-western-blot.jpg "Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] (ab223500)")
Key features and details
- Rabbit monoclonal [SP327] to ERK1 + ERK2 (phospho T202 + Y204)
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327]
See all ERK1 + ERK2 primary antibodies -
Description
Rabbit monoclonal [SP327] to ERK1 + ERK2 (phospho T202 + Y204) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ERK1 + ERK2 (phospho T202 + Y204).
Database link: P27361 -
Positive control
- WB: A431 + EGF cell lysates. IHC-P: Human breast ductal carcinoma, bladder transitional cell carcinoma and breast tissues. Flow Cyt: A431 + EGF cells.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Clone number
SP327 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] (ab223500)All lanes : Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] (ab223500) at 1/400 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) + EGF cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
Predicted band size: 42,44 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] (ab223500)](https://www.abcam.com/ps/products/223/ab223500/Images/ab223500-282997-anti-erk1-erk2-phospho-t202-y204-antibody-sp327-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] (ab223500)Paraffin-embedded human breast ductal carcinoma tissue stained for ERK1 + ERK2 (phospho T202 + Y204) using ab223500 at 1/100 dilution in immunohistochemical analysis.
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![Flow Cytometry - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] (ab223500)](https://www.abcam.com/ps/products/223/ab223500/Images/ab223500-282999-anti-erk1-erk2-phospho-t202-y204-antibody-sp327-flow-cytometry.jpg)
Flow Cytometry - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] (ab223500)Flow cytometric analysis of ab223500 in A431 (human epidermoid carcinoma cell line) cells + EGF (green) at 1/100 dilution compared to negative control of rabbit IgG (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] (ab223500)](https://www.abcam.com/ps/products/223/ab223500/Images/ab223500-282996-anti-erk1-erk2-phospho-t202-y204-antibody-sp327-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] (ab223500)Paraffin-embedded human breast tissue stained for ERK1 + ERK2 (phospho T202 + Y204) using ab223500 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] (ab223500)](https://www.abcam.com/ps/products/223/ab223500/Images/ab223500-282995-anti-erk1-erk2-phospho-t202-y204-antibody-sp327-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 (phospho T202 + Y204) antibody [SP327] (ab223500)Paraffin-embedded human bladder transitional cell carcinoma tissue stained for ERK1 + ERK2 (phospho T202 + Y204) using ab223500 at 1/100 dilution in immunohistochemical analysis.
