Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ubiquitin antibody [6C11-B3] (ab223368)

Key features and details

  • Mouse monoclonal [6C11-B3] to Ubiquitin
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Ubiquitin antibody [6C11-B3]
    See all Ubiquitin primary antibodies

  • Description

    Mouse monoclonal [6C11-B3] to Ubiquitin

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified) corresponding to Cow Ubiquitin conjugated to keyhole limpet haemocyanin.
    Database link: P0CG53

  • Positive control

    • ICC/IF: HeLa cells. WB: Mixture of human cell lines, cell lysate.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin antibody [6C11-B3] (ab223368)
    Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin antibody [6C11-B3] (ab223368)

    2% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Ubiquitin (green) using ab223368 at 1/100 dilution in ICC/IF.

    Secondary: Goat Anti-Mouse FITC at 1/200 dilution for 2 hours at RT.

    Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours RT.

    (A) DAPI nuclear stain (B) ab223368 (C) Merge.

  • Western blot - Anti-Ubiquitin antibody [6C11-B3] (ab223368)
    Western blot - Anti-Ubiquitin antibody [6C11-B3] (ab223368)
    Anti-Ubiquitin antibody [6C11-B3] (ab223368) at 1/1000 dilution + Human cell line mixture, cell lysate at 15 µg

    Secondary
    Sheep Anti-Mouse IgG HRP

    Predicted band size: 34 kDa
    Observed band size: 10 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 1.5% BSA.

    Primary antibody was incubated for 2 hours, the secondary for 1 hour at room temperature.

    The cell line mixture contains: A431 (human epidermoid carcinoma cell line), A549 (human lung carcinoma cell line), HCT116 (human colon cancer), HeLa (human epithelial cell line from cervix adenocarcinoma), HEK-293 (human epithelial cell line from embryonic kidney), HepG2 (human liver hepatocellular carcinoma cell line), HL-60 (human promyelocytic leukemia cell line), HUVEC (human umbilical vein endothelial cell line), Jurkat (human T cell leukemia cell line from peripheral blood), MCF7 (human breast adenocarcinoma cell line), PC-3 (human prostate adenocarcinoma cell line) and T98G (human brain glioblastoma).

  • Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin antibody [6C11-B3] (ab223368)
    Immunocytochemistry/ Immunofluorescence - Anti-Ubiquitin antibody [6C11-B3] (ab223368)

    2% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Ubiquitin (red) using ab223368 at 1/100 dilution in ICC/IF.

    Secondary: Goat Anti-Mouse APC at 1/200 dilution for 2 hours at RT.

    Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours RT.

    (A) DAPI nuclear stain (B) ab223368 (C) Merge.

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