Antibodies, Proteins, Kits and Reagents for Life Science

345 091 ₸ Product size

100 µl 345 091 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8590-448] to Ubiquitin (linkage-specific K63)
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Mouse, Rat, Human, Recombinant fragment

Product name

Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448]
See all Ubiquitin primary antibodies
Description

Rabbit monoclonal [EPR8590-448] to Ubiquitin (linkage-specific K63)
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Mouse Rat Human Recombinant fragment

See all applications and species data

Immunogen

Synthetic peptide within Human Ubiquitin aa 50-150 (linkage-specific K63) (Cysteine residue). The exact sequence is proprietary.
Database link: P0CG48
Positive control
- WB: K63-linked-Ub2-7 recombinant protein. HEk-293 and HeLa cell lysate. Mouse and rat brain lysate. IHC: Human kidney carcinoma tissue. Human tonsil tissue. Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR8590-448
Isotype

IgG
Research areas

Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)

All lanes : Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434) at 1/1000 dilution (purified)

Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 77 kDa
Observed band size: 16-300 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)

Immunohistochemical staining of paraffin embedded human tonsil with purified ab179434 at a dilution of 1/500. The secondary antibody used is a HRP conjugated goat anti-rabbit (ab97051, 1/500). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)

All lanes : Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434) at 1/5000 dilution (purified)

Lane 1 : K6-linked-Ub2 recombinant protein
Lane 2 : K11-linked-Ub2 recombinant protein
Lane 3 : K29-linked-Ub2 recombinant protein
Lane 4 : K33-linked-Ub2 recombinant protein
Lane 5 : K48-linked-Ub2 recombinant protein
Lane 6 : K63-linked-Ub2 recombinant protein

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 77 kDa
Observed band size: 16-70 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Flow Cytometry - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)

Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling Ubiquitin (linkage-specific K63) with purified ab179434 at 1/210 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluor^®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)

All lanes : Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434) at 1/1000 dilution (purified)

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 77 kDa
Observed band size: 16-300 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)

Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue labeling K63-linkage specific polyubiquitin with unpurified ab179434 at 1/100 dilution.
Western blot - Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)

All lanes : Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434) at 1/1000 dilution (unpurified)

Lane 1 : Ubiquitin recombinant protein
Lane 2 : K6-linked-Ub2 recombinant protein
Lane 3 : K11-linked-Ub2 recombinant protein
Lane 4 : K27-linked-Ub2 recombinant protein
Lane 5 : K29-linked-Ub2 recombinant protein
Lane 6 : K33-linked-Ub2 recombinant protein
Lane 7 : K48-linked-Ub2-7 recombinant protein
Lane 8 : K63-linked-Ub2-7 recombinant protein

Lysates/proteins at 0.02 µg per lane.

Predicted band size: 77 kDa
Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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