Antibodies, Proteins, Kits and Reagents for Life Science

251 280 ₸ Product size

100 µl 251 280 ₸

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Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2400] to Tau (phospho S198)
Suitable for: WB, IP, IHC-P
Reacts with: Human

Product name

Anti-Tau (phospho S198) antibody [EPR2400]
See all Tau primary antibodies
Description

Rabbit monoclonal [EPR2400] to Tau (phospho S198)
Host species

Rabbit
Specificity

ab79540 only detects Tau phosphorylated on serine 198.

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

corresponding to Human Tau. A phospho-specific peptide corresponding to residues surrounding serine 198 of Human Tau
Positive control
- SH-SY5Y cell lysates. Human brain tissue. IP: SH-SY5Y whole cell lysate.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR2400
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)

ab79540, at 1/100 dilution, staining Tau (phospho S198) in paraffin-embedded human brain tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)

All lanes : Anti-Tau (phospho S198) antibody [EPR2400] (ab79540) at 1/100000 dilution

Lane 1 : SH-SY5Y cell lysate
Lane 2 : SH-SY5Y cell lysate treated with AP

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 79 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?
Immunoprecipitation - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)

Purified ab79540 at 1/50 dilution (2µg) immunoprecipitating Tau in SH-SY5Y whole cell lysate.
Lane 1 (input): SY-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab79540 + SH-SY5Y whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab79540 in SH-SY5Y whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 50-70 kDa
Western blot - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540) Ercan E, Eid S, Weber C, et al., Mol Neurodegener., 12(1):87. Fig 6O.; doi:10.1186/s13024-017-0229-1. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

All lanes : Anti-Tau (phospho S198) antibody [EPR2400] (ab79540) at 1/100 dilution

Lanes 1-3 : hTg-Tau (transgenic mice expressing human tau protein) lysate
Lanes 4-6 : Tau-KO brain lysates

Predicted band size: 79 kDa
Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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