Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PER2 antibody [EPR11381(2)] - BSA and Azide free (ab238973)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11381(2)] to PER2 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human

Overview

  • Product name

    Anti-PER2 antibody [EPR11381(2)] - BSA and Azide free
    See all PER2 primary antibodies

  • Description

    Rabbit monoclonal [EPR11381(2)] to PER2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells.

  • General notes

    ab238973 is the carrier-free version of ab179813.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PER2 antibody [EPR11381(2)] - BSA and Azide free (ab238973)
    Immunocytochemistry/ Immunofluorescence - Anti-PER2 antibody [EPR11381(2)] - BSA and Azide free (ab238973)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PER2 with purified ab179813 at a dilution of 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/200) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179813).

  • Flow Cytometry - Anti-PER2 antibody [EPR11381(2)] - BSA and Azide free (ab238973)
    Flow Cytometry - Anti-PER2 antibody [EPR11381(2)] - BSA and Azide free (ab238973)

    Flow Cytometry analysis of HeLa cells labelling PER2 with purified ab179813 at a dilution of 1/200 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179813).

  • Flow Cytometry - Anti-PER2 antibody [EPR11381(2)] - BSA and Azide free (ab238973)
    Flow Cytometry - Anti-PER2 antibody [EPR11381(2)] - BSA and Azide free (ab238973)

    Flow cytometric analysis of permeabilized HeLa cells labeling PER2 with unpurified ab179813 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179813).

  • Immunocytochemistry/ Immunofluorescence - Anti-PER2 antibody [EPR11381(2)] - BSA and Azide free (ab238973)
    Immunocytochemistry/ Immunofluorescence - Anti-PER2 antibody [EPR11381(2)] - BSA and Azide free (ab238973)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling PER2 with unpurified ab179813 at a dilution of 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179813).

  • Immunocytochemistry/ Immunofluorescence - Anti-PER2 antibody [EPR11381(2)] - BSA and Azide free (ab238973)
    Immunocytochemistry/ Immunofluorescence - Anti-PER2 antibody [EPR11381(2)] - BSA and Azide free (ab238973)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PER2 with purified ab179813 at a dilution of 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/200) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab179813).

  • Anti-PER2 antibody [EPR11381(2)] - BSA and Azide free (ab238973)
    Anti-PER2 antibody [EPR11381(2)] - BSA and Azide free (ab238973)

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