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Signal Transduction Cytoskeleton / ECM Cell Adhesion Tight Junctions

Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (ab172968)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (ab172968)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10810] to Claudin 5 (phospho Y217)
  • Suitable for: WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-Claudin 5 (phospho Y217) antibody [EPR10810]
    See all Claudin 5 primary antibodies
  • Description

    Rabbit monoclonal [EPR10810] to Claudin 5 (phospho Y217)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Claudin 5 aa 200 to the C-terminus (phospho Y217). The exact sequence is proprietary.
    Database link: O00501

  • Positive control

    • MCF7 cell lysate, treated with pervanadate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10810
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Tight Junctions

Images

  • Western blot - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (ab172968)
    Western blot - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (ab172968)
    All lanes : Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (ab172968) at 1/50000 dilution

    Lane 1 : MCF7 cell lysate, untreated
    Lane 2 : MCF7 cell lysate, treated with pervanadate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 23 kDa

  • Immunoprecipitation - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (ab172968)
    Immunoprecipitation - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (ab172968)

    Western blot analysis on immunoprecipitation pellet from (1) MCF7 cells treated with pervanadate lysate or (2) 1X PBS (negative control) using ab172968 Phospho (pY217) at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (ab172968)
    Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (ab172968)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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