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Anti-Claudin 5 (phospho Y217) antibody [EPR10810] - BSA and Azide free (ab249739)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10810] to Claudin 5 (phospho Y217) - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Claudin 5 (phospho Y217) antibody [EPR10810] - BSA and Azide free
    See all Claudin 5 primary antibodies

  • Description

    Rabbit monoclonal [EPR10810] to Claudin 5 (phospho Y217) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249739 is the carrier-free version of ab172968. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249739 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR10810

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] - BSA and Azide free (ab249739)
    Western blot - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] - BSA and Azide free (ab249739)
    All lanes : Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (ab172968) at 1/50000 dilution

    Lane 1 : MCF7 cell lysate, untreated
    Lane 2 : MCF7 cell lysate, treated with pervanadate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 23 kDa



    This data was developed using ab172968, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] - BSA and Azide free (ab249739)
    Immunoprecipitation - Anti-Claudin 5 (phospho Y217) antibody [EPR10810] - BSA and Azide free (ab249739)
    This data was developed using ab172968, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from (1) MCF7 cells treated with pervanadate lysate or (2) 1X PBS (negative control) using ab172968 Phospho (pY217) at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
  • Anti-Claudin 5 (phospho Y217) antibody [EPR10810] - BSA and Azide free (ab249739)
    Anti-Claudin 5 (phospho Y217) antibody [EPR10810] - BSA and Azide free (ab249739)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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