Anti-TJP2/ZO2 antibody (ab224314)
Key features and details
- Rabbit polyclonal to TJP2/ZO2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TJP2/ZO2 antibody
See all TJP2/ZO2 primary antibodies -
Description
Rabbit polyclonal to TJP2/ZO2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Dog -
Immunogen
Recombinant fragment corresponding to Human TJP2/ZO2 aa 300-500.
Database link: Q9UDY2 -
Positive control
- WB: A431 cell lysate. ICC/IF: U-2 OS cells. IHC-P: Human colon tissue.
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General notes
This product was previously labelled as TJP2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : ab224314 at 0.4 µg/ml
Lane 1 : Human A-431 cell lines
Lane 2 : Human SK-MEL-30 cell line
Predicted band size: 134 kDaLoading control: Anti-HSP90B1
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Paraffin-embedded human testis stained for TJP2 using ab224314 at 1/50 dilution in immunohistochemical analysis. Shows strong cytoplasmic and membranous positivity in cells in seminiferous ducts.
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PFA-fixed/Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for TJP2/ZO2 (green) using ab224314 (4 µg/ml) in ICC/IF.
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Paraffin-embedded human pancreas stained for TJP2 using ab224314 at 1/50 dilution in immunohistochemical analysis. Shows weak cytoplasmic positivity in exocrine glandular cells.
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Paraffin-embedded human cerebral cortex stained for TJP2 using ab224314 at 1/50 dilution in immunohistochemical analysis. Shows strong cytoplasmic positivity in glial cells.
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Paraffin-embedded human colon stained for TJP2 using ab224314 at 1/50 dilution in immunohistochemical analysis. Shows moderate cytoplasmic and membranous positivity in glandular cells.