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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins

Anti-STUB1/CHIP antibody (ab229108)

Anti-STUB1/CHIP antibody (ab229108)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to STUB1/CHIP
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-STUB1/CHIP antibody
    See all STUB1/CHIP primary antibodies
  • Description

    Rabbit polyclonal to STUB1/CHIP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human STUB1/CHIP (internal sequence). The exact sequence is proprietary.
    Database link: Q9UNE7

  • Positive control

    • WB: HEK-293T whole cell lysate; Neuro-2a, C8D30, NIH/3T3, RAW 264.7, C2C12, PC-12 and Rat2 whole cell extracts. IHC-P: Human breast cancer tissue.
  • General notes

    Previously labelled as STUB1.  

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • SMADs
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Ubiquitin ligases
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • E3 Ubiquitin Ligases

Images

  • Western blot - Anti-STUB1/CHIP antibody (ab229108)
    Western blot - Anti-STUB1/CHIP antibody (ab229108)
    Anti-STUB1/CHIP antibody (ab229108) at 1/10000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Predicted band size: 35 kDa



    10% SDS-PAGE gel.

  • Western blot - Anti-STUB1/CHIP antibody (ab229108)
    Western blot - Anti-STUB1/CHIP antibody (ab229108)
    All lanes : Anti-STUB1/CHIP antibody (ab229108) at 1/10000 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
    Lane 2 : C8D30 whole cell extract
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
    Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
    Lane 5 : C2C12 (mouse myoblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 35 kDa



    12% SDS-PAGE gel.

  • Western blot - Anti-STUB1/CHIP antibody (ab229108)
    Western blot - Anti-STUB1/CHIP antibody (ab229108)
    All lanes : Anti-STUB1/CHIP antibody (ab229108) at 1/10000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 35 kDa



    12% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STUB1/CHIP antibody (ab229108)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STUB1/CHIP antibody (ab229108)

    Paraffin-embedded human breast cancer tissue stained for STUB1/CHIP using ab229108 at 1/500 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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