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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins

Anti-STUB1/CHIP antibody (ab228742)

Anti-STUB1/CHIP antibody (ab228742)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to STUB1/CHIP
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-STUB1/CHIP antibody
    See all STUB1/CHIP primary antibodies
  • Description

    Rabbit polyclonal to STUB1/CHIP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Rhesus monkey, Bovine papillomavirus
  • Immunogen

    Synthetic peptide within Human STUB1/CHIP (C terminal). The exact sequence is proprietary. Carrier-protein conjugated.
    Database link: Q9UNE7

  • Positive control

    • WB: HEK-293T and HepG2 whole cell lysate. IHC-P: Mouse esophagus tissue; ES2 xenograft tissue. ICC/IF: HeLa cells.
  • General notes

    Previously labelled as STUB1.  

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • SMADs
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Ubiquitin ligases
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • E3 Ubiquitin Ligases

Images

  • Western blot - Anti-STUB1/CHIP antibody (ab228742)
    Western blot - Anti-STUB1/CHIP antibody (ab228742)
    All lanes : Anti-STUB1/CHIP antibody (ab228742) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 35 kDa



    10% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STUB1/CHIP antibody (ab228742)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STUB1/CHIP antibody (ab228742)

    Paraffin-embedded mouse esophagus tissue stained for STUB1/CHIP using ab228742 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STUB1/CHIP antibody (ab228742)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STUB1/CHIP antibody (ab228742)

    Paraffin-embedded ES2 xenograft tissue stained for STUB1/CHIP using ab228742 at 1/500 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-STUB1/CHIP antibody (ab228742)
    Immunocytochemistry/ Immunofluorescence - Anti-STUB1/CHIP antibody (ab228742)

    Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for STUB1/CHIP (green) using ab228742 at 1/200 dilution in ICC/IF.

    Nuclear counterstain: DNA probe (blue).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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