Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866)
![Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866)](https://www.abcam.com/ps/products/181/ab181866/Images/ab181866-216174-anti-alpha-a-crystallin-antibody-epr14125b-immunofluorescence.jpg "Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14125(B)] to alpha A Crystallin/CRYAA
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)]
See all alpha A Crystallin/CRYAA primary antibodies -
Description
Rabbit monoclonal [EPR14125(B)] to alpha A Crystallin/CRYAA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Recombinant human alpha A Crystallin/CRYAA protein (ab48778) can be used as a positive control in WB. Rat and Mouse eyeball. Y79 cells.
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General notes
This product was previously labelled as alpha A Crystallin
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14125(B) -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866)
Immunofluorescence analysis of Y79 cells fixed with acetone labeling alpha A Crystallin/CRYAA with ab181866 at 1/500 dilution followed by Goat anti rabbit IgG(Alexa Fluor®555) at 1/200 dilution and counterstained with Dapi.
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![Western blot - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866)](https://www.abcam.com/ps/products/181/ab181866/Images/ab181866-216173-anti-alpha-a-crystallin-antibody-epr14125b-western-blot.jpg)
Western blot - Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866)All lanes : Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866) at 1/2000 dilution
Lane 1 : Rat eyeball
Lane 2 : Mouse eyeball
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa
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![Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866)](https://www.abcam.com/ps/products/181/ab181866/Images/ab181866-3-benefits-of-recombinant-antibodies.png)
Anti-alpha A Crystallin/CRYAA antibody [EPR14125(B)] (ab181866)
