Anti-Hsp27 (phospho S86) antibody (ab17938)
Key features and details
- Rabbit polyclonal to Hsp27 (phospho S86)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Hsp27 (phospho S86) antibody
See all Hsp27 primary antibodies -
Description
Rabbit polyclonal to Hsp27 (phospho S86) -
Host species
Rabbit -
Specificity
Lysates prepared from NIH3T3 cells were immunoblotted in the presence of non-phosphopeptide corresponding to the immunogen, a generic phosphoserine-containing peptide or the phosphopeptide immunogen. The data show that only the peptide corresponding to the mouse phospho S86 HSP27 blocks the antibody signal, thereby demonstrating the specificity of the antibody. The signal was completely removed by lambda phosphatase treatment demonstrating that the antibody interacts specifically with the phosphorylated protein. -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic phosphopeptide derived from a region of mouse HSP25 that contains serine 86.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated HSP25 (the mouse homolog of human HSP27). The final product is generated by affinity chromatography using an HSP25-derived peptide that is phosphorylated at serine 86. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Hsp27 (phospho S86) antibody (ab17938)
Hsp25 (phospho S86) antibody image 6534.
Western blot using ab17938 on NIH3T3 cells treated with anisomycin.
Lane 1: unstimulated cells
Lane 2: cells stimulated with anisomycin
Lane 3: cells stimulated with anisomycin. Antibody blocked with the non-phosphopeptide corresponding to the immunogen
Lane 4: cells stimulated with anisomycin. Antibody blocked with generic phosphoserine-containing peptide
Lane 5: cells stimulated with anisomycin. Antibody blocked with the phosphopeptide immunogen
Lane 6: cells stimulated with anisomycin and treated with lambda phosphatase
10-30µ g of cell lysate can be loaded when using similar lysates with this antibody. Samples were run using SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with ab17938 for one hour at room temperature in 3% BSA-TBST buffer, following prior incubation with blocking
