Human HSP70 ELISA Kit (ab187399)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.5 U/ml
- Range: 7.81 U/ml - 500 U/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human HSP70 ELISA Kit
See all HSP70 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 3.6% Inter-assay Sample n Mean SD CV% Overall 3 6.1% -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
2.5 U/ml -
Range
7.81 U/ml - 500 U/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 90.8 79.7% - 96.6% Tissue Culture Media 69.5 67.6% - 70.5% Goat Serum 67.9 67% - 68.6% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human HSP70 ELISA Kit (ab187399) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of HSP70 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human HSP70 with 2.5 U/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
HSP70 belongs to the heat shock protein 70 family. In cooperation with other chaperones, HSP70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, HSP70 serves as a post-attachment receptor for the virus to facilitate entry into the cell. HSP70 is induced by heat shock.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human HSP70 Capture Antibody 1 x 600µl 10X Human HSP70 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human HSP70 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Cellular localization
Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs -
Alternative names
- DnaK type molecular chaperone HSP70 1
- Heat shock 70 kDa protein 1/2
- Heat shock 70 kDa protein 1A/1B
see all -
Database links
- Entrez Gene: 3303 Human
- Omim: 140550 Human
- SwissProt: P08107 Human
- SwissProt: P0DMV8 Human
- SwissProt: P0DMV9 Human
Images
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Other - Human HSP70 ELISA Kit (ab187399)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of HSP70 standard curveBackground-subtracted data values (mean +/- SD) are graphed.
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Titration of HeLa cell extract within the working range of the assayBackground-subtracted data values (mean +/- SD, n=2) are graphed.
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Titration of Human tissue homogenate extracts within the working range of the assayBackground-subtracted data values (mean +/- SD, n=2) are graphed.
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HSP70 concentrations in un-induced and heat shock induced cellsCell extracts (250 µg/mL) prepared from un-induced (control) and heat-shocked (HS, 30 min at 42ºC, followed by recovery period, as indicated, at 37ºC) cells were analyzed with this kit. The concentrations of HSP70 were interpolated from data values using HSP70 standard curve and graphed in units (U) of HSP70 per mg of extract (mean, n=2).
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HSP70 capture antibody specificity by Western blot analysisCell extracts (40 µg) prepared from un-induced (lane 3, Jurkat; lane 7, HeLa) cells or 30 min at 42ºC heat-shocked, then recovered at 37ºC for variable time periods (lane 4, 1 hour; lane 5, 4 hours; lane 6, 6.5 hours) Jurkat cells were analyzed by Western blotting using the capture antibody of this kit. The Human recombinant HSP70 protein standard was analyzed as well (lane 2). Note that this antibody detects a single band of 70 kDa and that the HSP70 levels obtained by this analysis correlate with the result obtained with the use of this kit.
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HSP70 detector antibody specificity by Western blot analysisCell extracts (40 µg) prepared from un-induced (lane 3, Jurkat; lane 7, HeLa) cells or 30 min at 42ºC heat-shocked, then recovered at 37ºC for variable time periods (lane 4, 1 hour; lane 5, 4 hours; lane 6, 6.5 hours) Jurkat cells were analyzed by Western blotting using the detector antibody of this kit. The Human recombinant HSP70 protein standard was analyzed as well (lane 2). Note that this antibody detects a single band of 70 kDa and that the HSP70 levels obtained by this analysis correlate with the result obtained with the use of this kit.
