Anti-PBR antibody (ab154878)
Key features and details
- Rabbit polyclonal to PBR
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
-
Product name
Anti-PBR antibody
See all PBR primary antibodies -
Description
Rabbit polyclonal to PBR -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Horse, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
-
Immunogen
Synthetic peptide within Rat PBR aa 1-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P16257 -
Positive control
- This antibody gave a positive result in IHC in the following FFPE tissue: Normal Rat Testes
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab154878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Target
-
Function
Responsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells. -
Tissue specificity
Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids. -
Sequence similarities
Belongs to the TspO/BZRP family. -
Cellular localization
Mitochondrion membrane. - Information by UniProt
-
Database links
- Entrez Gene: 706 Human
- Entrez Gene: 12257 Mouse
- Entrez Gene: 24230 Rat
- Omim: 109610 Human
- SwissProt: B1AH88 Human
- SwissProt: P30536 Human
- SwissProt: P50637 Mouse
- SwissProt: P16257 Rat
see all -
Alternative names
- Benzodiazapine receptor (peripheral) antibody
- Benzodiazepine peripheral binding site antibody
- BPBS antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBR antibody (ab154878)
IHC image of TPBR staining in normal Rat testes formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab154878, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
References (1)
ab154878 has been referenced in 1 publication.
- Owen DR et al. TSPO mutations in rats and a human polymorphism impair the rate of steroid synthesis. Biochem J 474:3985-3999 (2017). WB ; Rat . PubMed: 29074640
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBR antibody (ab154878)
IHC image of TPBR staining in normal Rat testes formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab154878, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
