Alexa Fluor® 647 Anti-PBR antibody [EPR5384] (ab199836)
![Alexa Fluor® 647 Anti-PBR antibody [EPR5384] (ab199836)](https://www.abcam.com/ps/products/199/ab199836/Images/ab199836-283637-anti-pbr-antibody-epr5384-alexa-fluor-647-immunocytochemistry.jpg "Alexa Fluor® 647 Anti-PBR antibody [EPR5384] (ab199836)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR5384] to PBR
- Suitable for: ICC
- Knockout validated
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Overview
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Product name
Alexa Fluor® 647 Anti-PBR antibody [EPR5384]
See all PBR primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EPR5384] to PBR -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab170987) -
Positive control
- ICC/IF: HeLa cells, HAP1-TSPO cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.012% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR5384 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Alexa Fluor® 647 Anti-PBR antibody [EPR5384] (ab199836)
ab199836 staining PBR in wild-type HAP1 cells (top panel) and PBR knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab199836 at 1/500 dilution (shown in red) and ab195887at 1/250 dilution (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Immunocytochemistry - Alexa Fluor® 647 Anti-PBR antibody [EPR5384] (ab199836)](https://www.abcam.com/ps/products/199/ab199836/Images/ab199836-242623-anti-pbr-antibody-epr5384-alexa-fluor-647-immunocytochemistry.jpg)
Immunocytochemistry - Alexa Fluor® 647 Anti-PBR antibody [EPR5384] (ab199836)ab199836 staining PBR in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Alexa Fluor® 647 Anti-PBR antibody [EPR5384] (ab199836)](https://www.abcam.com/ps/products/199/ab199836/Images/ab199836-4-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 647 Anti-PBR antibody [EPR5384] (ab199836)
