Anti-PBR antibody - C-terminal (ab226783)
Key features and details
- Rabbit polyclonal to PBR - C-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PBR antibody - C-terminal
See all PBR primary antibodies -
Description
Rabbit polyclonal to PBR - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human PBR (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: P30536 -
Positive control
- WB: HeLa cell extract; U-87 MG and A431 whole cell extracts. IP: A431 whole cell extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PBR antibody - C-terminal (ab226783)All lanes : Anti-PBR antibody - C-terminal (ab226783) at 1/5000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : PBR Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 19 kDa
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Western blot - Anti-PBR antibody - C-terminal (ab226783)All lanes : Anti-PBR antibody - C-terminal (ab226783) at 1/5000 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : PBR knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 19 kDa15% SDS-PAGE gel.
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Immunoprecipitation - Anti-PBR antibody - C-terminal (ab226783)PBR was immunoprecipitated from A431 (human epidermoid carcinoma cell line) whole cell extract with 5 µg ab226783. Western blot was performed from the immunoprecipitate using ab226783.
Lane 1: Control IgG IP in A431 whole cell extract.
Lane 2: ab226783 IP in A431 whole cell extract.
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Western blot - Anti-PBR antibody - C-terminal (ab226783)All lanes : Anti-PBR antibody - C-terminal (ab226783) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 19 kDa15% SDS-PAGE gel.
