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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Proteasome

Anti-PSME1 antibody (ab236877)

Anti-PSME1 antibody (ab236877)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PSME1
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PSME1 antibody
    See all PSME1 primary antibodies
  • Description

    Rabbit polyclonal to PSME1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig, Cynomolgus monkey
  • Immunogen

    Recombinant full length protein corresponding to Human PSME1 aa 1 to the C-terminus.
    Database link: Q06323-1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Mouse liver tissue lysate. Raji whole cell lysate. IHC-P: Human liver cancer and testis tissues. IP: Jurkat whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome

Images

  • Western blot - Anti-PSME1 antibody (ab236877)
    Western blot - Anti-PSME1 antibody (ab236877)
    All lanes : Anti-PSME1 antibody (ab236877) at 1/500 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution

    Predicted band size: 29 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME1 antibody (ab236877)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME1 antibody (ab236877)

    Paraffin-embedded human testis tissue stained for PSME1 using ab236877 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME1 antibody (ab236877)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME1 antibody (ab236877)

    Paraffin-embedded human liver cancer tissue stained for PSME1 using ab236877 at 1/100 dilution in immunohistochemical analysis.

  • Immunoprecipitation - Anti-PSME1 antibody (ab236877)
    Immunoprecipitation - Anti-PSME1 antibody (ab236877)

    PSME1 was immunoprecipitated from Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate using ab236877 at 1/200 dilution.

    Lane 1: Rabbit control IgG in Jurkat whole cell lysate.

    Lane 2: ab236877 in Jurkat whole cell lysate.

    Lane 3: Jurkat whole cell lysate (10 µg input).

    For western blotting an HRP-conjugated Protein G antibody was used as the secondary antibody at a 1/2000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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