Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PSME1 antibody [EPR10967(B)] (ab155091)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10967(B)] to PSME1
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-PSME1 antibody [EPR10967(B)]
    See all PSME1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10967(B)] to PSME1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PSME1. The exact sequence is proprietary.

  • Positive control

    • WB: HEK293T, MCF7 and Jurkat lysates. ICC: MCF7 and HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10967(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091)
    Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091)
    All lanes : Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : PSME1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab155091 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab155091 Anti-PSME1 antibody [EPR10967(B)] was shown to specifically react with PSME1 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266192 (knockout cell lysate ab257613) was used. Wild-type and PSME1 knockout samples were subjected to SDS-PAGE. ab155091 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (ab155091)
    Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (ab155091)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling PSME1 with purified ab155091 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor®488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091)
    Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091)
    All lanes : Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 29 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (ab155091)
    Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (ab155091)
    Immunofluorescent analysis of MCF7 cells labeling PSME1 with ab155091 at 1/100 dilution.
  • Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091)
    Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091) This image is courtesy of an anonymous Abreview
    All lanes : Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution

    All lanes : Human prostate cancer whole tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 29 kDa


    Exposure time: 2 minutes

    See Abreview

  • Anti-PSME1 antibody [EPR10967(B)] (ab155091)
    Anti-PSME1 antibody [EPR10967(B)] (ab155091)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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