Anti-DDX39 antibody [2252C4a] (ab50697)
![Anti-DDX39 antibody [2252C4a] (ab50697)](https://www.abcam.com/ps/products/50/ab50697/Images/ab50697-62369-anti-ddx39-antibody-2252c4a-western-blot.jpg "Anti-DDX39 antibody [2252C4a] (ab50697)")
Key features and details
- Mouse monoclonal [2252C4a] to DDX39
- Suitable for: WB, IP
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-DDX39 antibody [2252C4a]
See all DDX39 primary antibodies -
Description
Mouse monoclonal [2252C4a] to DDX39 -
Host species
Mouse -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment -
Immunogen
Recombinant fragment
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Positive control
- WB: HeLa, NIH3T3, F2480 whole cell lysate. IP: HeLa cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane. -
Clonality
Monoclonal -
Clone number
2252C4a -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-DDX39 antibody [2252C4a] (ab50697)Anti-DDX39 antibody [2252C4a] (ab50697) + immunized recombinant protein
Predicted band size: 49 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
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![Western blot - Anti-DDX39 antibody [2252C4a] (ab50697)](https://www.abcam.com/ps/products/50/ab50697/Images/ab50697-62368-anti-ddx39-antibody-2252c4a-western-blot.jpg)
Western blot - Anti-DDX39 antibody [2252C4a] (ab50697)Anti-DDX39 antibody [2252C4a] (ab50697) at 1/100 dilution + HeLa whole cell lysate at 25 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 49 kDa
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![Immunoprecipitation - Anti-DDX39 antibody [2252C4a] (ab50697)](https://www.abcam.com/ps/products/50/ab50697/Images/ab50697-62367-anti-ddx39-antibody-2252c4a-immunoprecipitation.jpg)
Immunoprecipitation - Anti-DDX39 antibody [2252C4a] (ab50697)500µg HeLa cell lysate was prepared in RIPA buffer and immunoprecipitated with 5µg ab50697. The subsequent Western blot was probed with ab50697 at 1/50, followed by TrueBlot anti-mouse IgG at 1/1000. Developed by the ECL technique. -
![Western blot - Anti-DDX39 antibody [2252C4a] (ab50697)](https://www.abcam.com/ps/products/50/ab50697/Images/ab50697-62366-anti-ddx39-antibody-2252c4a-western-blot.jpg)
Western blot - Anti-DDX39 antibody [2252C4a] (ab50697)Anti-DDX39 antibody [2252C4a] (ab50697) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 49 kDa
-
![Western blot - Anti-DDX39 antibody [2252C4a] (ab50697)](https://www.abcam.com/ps/products/50/ab50697/Images/ab50697-62365-anti-ddx39-antibody-2252c4a-western-blot.jpg)
Western blot - Anti-DDX39 antibody [2252C4a] (ab50697)Anti-DDX39 antibody [2252C4a] (ab50697) at 1/100 dilution + F2480 whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 49 kDa
