Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DDX39 antibody [EPR13507(B)] (ab176348)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13507(B)] to DDX39
  • Suitable for: WB, ICC/IF, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-DDX39 antibody [EPR13507(B)]
    See all DDX39 primary antibodies

  • Description

    Rabbit monoclonal [EPR13507(B)] to DDX39

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human DDX39 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: O00148

  • Positive control

    • WB: Human seminoma, Jurkat, HeLa, HepG2 and A431 cell lysates. IHC-P: Human colon and kidney tissues. ICC/IF: HepG2 cells. IP: Jurkat whole cell lysate (ab7899).

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-DDX39 antibody [EPR13507(B)] (ab176348)
    Western blot - Anti-DDX39 antibody [EPR13507(B)] (ab176348)
    All lanes : Anti-DDX39 antibody [EPR13507(B)] (ab176348) at 1/10000 dilution

    Lane 1 : Human seminoma lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 49 kDa



    Western blot analysis on immunoprecipitation pellet from (1) Jurkat cells lysate or (2) 1X PBS (negative control) using ab176348 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13507(B)] (ab176348)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13507(B)] (ab176348)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling DDX39 with ab176348 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-DDX39 antibody [EPR13507(B)] (ab176348)
    Immunocytochemistry/ Immunofluorescence - Anti-DDX39 antibody [EPR13507(B)] (ab176348)

    Immunofluorescent staining of HepG2 cells labeling DDX39 with ab176348 at 1/50 dilution.

  • Immunoprecipitation - Anti-DDX39 antibody [EPR13507(B)] (ab176348)
    Immunoprecipitation - Anti-DDX39 antibody [EPR13507(B)] (ab176348)

    Western blot analysis on immunoprecipitation pellet from (1) Jurkat cells lysate or (2) 1X PBS (negative control) using ab176348 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13507(B)] (ab176348)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13507(B)] (ab176348)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DDX39 with ab176348 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-DDX39 antibody [EPR13507(B)] (ab176348)
    Anti-DDX39 antibody [EPR13507(B)] (ab176348)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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