Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-DDX39 antibody [EPR13507(B)] (ab176348)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13507(B)] to DDX39
Suitable for: WB, ICC/IF, IP, IHC-P
Reacts with: Human

Product name

Anti-DDX39 antibody [EPR13507(B)]
See all DDX39 primary antibodies
Description

Rabbit monoclonal [EPR13507(B)] to DDX39
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human DDX39 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: O00148
Positive control
- WB: Human seminoma, Jurkat, HeLa, HepG2 and A431 cell lysates. IHC-P: Human colon and kidney tissues. ICC/IF: HepG2 cells. IP: Jurkat whole cell lysate (ab7899).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13507(B)
Isotype

IgG
Research areas

Western blot - Anti-DDX39 antibody [EPR13507(B)] (ab176348)

All lanes : Anti-DDX39 antibody [EPR13507(B)] (ab176348) at 1/10000 dilution

Lane 1 : Human seminoma lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : A431 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 49 kDa

Western blot analysis on immunoprecipitation pellet from (1) Jurkat cells lysate or (2) 1X PBS (negative control) using ab176348 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13507(B)] (ab176348)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling DDX39 with ab176348 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-DDX39 antibody [EPR13507(B)] (ab176348)

Immunofluorescent staining of HepG2 cells labeling DDX39 with ab176348 at 1/50 dilution.
Immunoprecipitation - Anti-DDX39 antibody [EPR13507(B)] (ab176348)

Western blot analysis on immunoprecipitation pellet from (1) Jurkat cells lysate or (2) 1X PBS (negative control) using ab176348 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13507(B)] (ab176348)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DDX39 with ab176348 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-DDX39 antibody [EPR13507(B)] (ab176348)

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