Arf6 Activation Assay Kit (ab211171)
Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
-
Product name
Arf6 Activation Assay Kit -
Sample type
Tissue, Adherent cells, Suspension cells -
Species reactivity
Reacts with: Rat, Cow, Dog, Human -
Product overview
Arf6 Activation Assay Kit (ab211171) uses GGA3 PBD Agarose beads to selectively isolate and pull-down the active form of Arf from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Arf is detected by western blot analysis using an anti-Arf6 antibody, which detects the human, rat, cow and dog protein.
Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.
-
Notes
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.
Arf6 (ADP-ribosylation factor 6) is a 20 kDa protein, member of ARF gene family, which constitutes part of the Ras superfamily. The Arf proteis are categorized as class I (Arf1, Arf2, Arf3), class II (Arf4 and Arf5) and class III (Arf6), and they all share a common gene organization. Arf6 regulates a variety of biological response pathways that include protein trafficking, organelle structure, and cytoskeleton remodeling. Like other small GTPases, Arf6 cycles between an inactive GDP-bound form and an active GTP-bound form: in its active (GTP-bound) state, Arf1 binds specifically to the protein-binding domain (PBD) of GGA3 to control downstream signaling cascades.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 20 tests ab211180 - GGA3 PBD Agarose Beads 1 x 200µl 100X GTPyS 1 x 20µl 100X GDP 1 x 20µl 5X Assay/Lysis Buffer 4 x 2ml anti-Arf6 Mouse monoclonal antibody 1 x 10µl GTPase immunoblot Positive Control 1 x 100µl -
Research areas
-
Function
GTP-binding protein involved in protein trafficking; regulates endocytic recycling and cytoskeleton remodeling. May modulate vesicle budding and uncoating within the Golgi apparatus. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in the regulation of dendritic spine development (By similarity). Contributes to the regulation of dendritic branching and filopodia extension. -
Sequence similarities
Belongs to the small GTPase superfamily. Arf family. -
Cellular localization
Golgi apparatus. Cell membrane. Endosome membrane. Cell membrane. Cell projection > filopodium membrane. Recruited to the cell membrane in association with CYTH2 and ARL4C. - Information by UniProt
-
Alternative names
- ADP ribosylation factor 6
- ADP ribosylation factor protein 6
- ADP-ribosylation factor 6
see all -
Database links
- Entrez Gene: 382 Human
- Entrez Gene: 79121 Rat
- Omim: 600464 Human
- SwissProt: P62330 Human
- SwissProt: P62332 Rat
- Unigene: 525330 Human
- Unigene: 719973 Human
- Unigene: 6993 Rat
Images
-
Arf6 Activation Assay Kit (ab211171)
Arf6 activation assay. Lane 1. HeLa cell lysate loaded with GDP and incubated with GGA3 PBD Agarose beads. Lane 2. HeLa cell lysate loaded with GTPγS and incubated with GGA3 PBD Agarose beads.
-
Arf6 Activation Assay Kit (ab211171)Arf6 positive control (293 cell lysate at 0.5 mg/mL) detected by immunoblot.